Design and Analysis of a Mutant Form of the Ice-binding Protein From Choristoneura Fumiferana
Document Type
Article
Publication Date
2022
Keywords
Ice-binding Protein, Ice Melting Temperature, Freezing Point of Water, Ice-binding Mutant Protein
Digital Object Identifier (DOI)
https://doi.org/10.1007/s10930-022-10049-6
Abstract
Ice-binding proteins are expressed in the cells of some cold adapted organisms, helping them to survive at extremely low temperatures. One of the problems in studying such proteins is the difficulty of their isolation and purification. For example, eight cysteine residues in the cfAF (antifreeze protein from the eastern spruce budworm Choristoneura fumiferana) form intermolecular bridges during the overexpression of this protein. This impedes the process of the protein purification dramatically. To overcome this issue, in this work, we designed a mutant form of the ice-binding protein cfAFP, which is much easier to isolate that the wild-type protein. The mutant form named mIBP83 did not lose the ability to bind to ice surface. Besides, observation of the processes of freezing and melting of ice in the presence of mIBP83 showed that this protein affects the process of ice melting, increasing its melting temperature, and does not decrease the water freezing temperature.
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
The Protein Journal, v. 41, p. 304-314
Scholar Commons Citation
Deeva, Anna A.; Glukhova, Ksenia A.; Isoyan, Lala S.; Okulova, Yuliya D.; Uversky, Vladimir N.; and Melnik, Bogdan S., "Design and Analysis of a Mutant Form of the Ice-binding Protein From Choristoneura Fumiferana" (2022). Molecular Medicine Faculty Publications. 969.
https://digitalcommons.usf.edu/mme_facpub/969
