On the Potential Significance of the Intrinsically Disordered Regions in The Clostridiodes Difficile Toxins A and B
Document Type
Article
Publication Date
2022
Keywords
Bacterial Toxin, Binding Promiscuity, Intrinsic Disorder, Multifunctionality, Protein-protein Interactions, Tcda, Tcdb
Digital Object Identifier (DOI)
http://dx.doi.org/10.2174/1389203723666220518111801
Abstract
Background: Clostridiodes (or Clostridium) difficile is a spore-forming, Gram-positive anaerobic bacterium that may cause symptoms ranging from diarrhea to pseudomembranous colitis. During the C. difficile infection (CDI), the two primary bacterial toxins, toxin A (TcdA) or toxin B (TcdB), disrupt host cell function mainly through the inactivation of small GTPases that regulate the actin cytoskeleton. Both toxins have complex structural organization containing several functional domains.
Methods: Analytical bioinformatics tools are used to compare the extent of disorder within TcdA and TcdB proteins, and to see if the existence of structural disorder can be used to explain the difference in the functionality of these toxins.
Results: This paper’s aim is to offer an overall review of the structural and functional differences between TcdA and TcdB.
Conclusion: Results of our multifactorial bioinformatics analysis revealed that intrinsic disorder may play a role in the multifunctionality of C. difficile major toxins TcdA and TcdB, suggesting that intrinsic disorder may be related to their pathogenic mechanisms.
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
Current Protein and Peptide Science, v. 23, issue 3, p. 192-209
Scholar Commons Citation
Hadidy, Nashwa El; Uversky, Vladimir N.; and Sun, Xingmin, "On the Potential Significance of the Intrinsically Disordered Regions in The Clostridiodes Difficile Toxins A and B" (2022). Molecular Medicine Faculty Publications. 962.
https://digitalcommons.usf.edu/mme_facpub/962
