Conservation of Intrinsic Diconservation of Intrinsic Disorder in Protein Domains and Families: II. Functions of Conserved Disordersorder in Protein Domains and Families: II. Functions of Conserved Disorder
Document Type
Article
Publication Date
2006
Keywords
Intrinsic Disorder, Protein Structure-function, Disorder Prediction, PONDR
Digital Object Identifier (DOI)
https://doi.org/10.1021/pr060049p
Abstract
Regions of conserved disorder prediction (CDP) were found in protein domains from all available InterPro member databases, although with varying frequency. These CDP regions were found in proteins from all kingdoms of life, including viruses. However, eukaryotes had 1 order of magnitude more proteins containing long disordered regions than did archaea and bacteria. Sequence conservation in CDP regions varied, but was on average slightly lower than in regions of conserved order. In some cases, disordered regions evolve faster than ordered regions, in others they evolve slower, and in the rest they evolve at roughly the same rate. A variety of functions were found to be associated with domains containing conserved disorder. The most common were DNA/RNA binding, and protein binding. Many ribosomal proteins also were found to contain conserved disordered regions. Other functions identified included membrane translocation and amino acid storage for germination. Due to limitations of current knowledge as well as the methodology used for this work, it was not determined whether these functions were directly associated with the predicted disordered region. However, the functions associated with conserved disorder in this work are in agreement with the functions found in other studies to correlate to disordered regions. We have established that intrinsic disorder may be more common in bacterial and archaeal proteins than previously thought, but this disorder is likely to be used for different purposes than in eukaryotic proteins, as well as occurring in shorter stretches of protein. Regions of predicted disorder were found to be conserved within a large number of protein families and domains. Although many think of such conserved domains as being ordered, in fact a significant number of them contain regions of disorder that are likely to be crucial to their functions.
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
Journal of Proteome Research, v. 5, issue 4, p. 888-898
Scholar Commons Citation
Chen, Jessica Walton; Romero, Pedro R.; Uversky, Vladimir N.; and Dunker, A. Keith, "Conservation of Intrinsic Diconservation of Intrinsic Disorder in Protein Domains and Families: II. Functions of Conserved Disordersorder in Protein Domains and Families: II. Functions of Conserved Disorder" (2006). Molecular Medicine Faculty Publications. 757.
https://digitalcommons.usf.edu/mme_facpub/757
