Document Type
Article
Publication Date
2002
Digital Object Identifier (DOI)
https://doi.org/10.1074/jbc.M109541200
Abstract
The pathological hallmark of Parkinson's disease is the presence of intracellular inclusions, Lewy bodies, and Lewy neurites, in the dopaminergic neurons of the substantia nigra and several other brain regions. Filamentous α-synuclein is the major component of these deposits and its aggregation is believed to play an important role in Parkinson's disease and several other neurodegenerative diseases. Two homologous proteins, β- and γ-synucleins, are also abundant in the brain. The synucleins are natively unfolded proteins. β-Synuclein, which lacks 11 central hydrophobic residues compared with its homologs, exhibited the properties of a random coil, whereas α- and γ-synucleins were slightly more compact and structured. γ-Synuclein, unlike its homologs, formed a soluble oligomer at relatively low concentrations, which appears to be an off-fibrillation pathway species. Here we show that, although they have similar biophysical properties to α-synuclein, β- And γ-synucleins inhibit α-synuclein fibril formation. Complete inhibition of α-synuclein fibrillation was observed at 4:1 molar excess of β- and γ-synucleins. No significant incorporation of β-synuclein into the fibrils was detected. The lack of fibrils formed by β-synuclein is most readily explained by the absence of a stretch of hydrophobic residues from the middle region of the protein. A model for the inhibition is proposed.
Rights Information
This work is licensed under a Creative Commons Attribution 4.0 License.
Was this content written or created while at USF?
No
Citation / Publisher Attribution
Journal of Biological Chemistry, v. 277, issue 14, p. 11970-11978
Scholar Commons Citation
Uversky, Vladimir N.; Li, Jie; Souillac, Pierre O.; Millett, Ian S.; Doniach, Sebastian; Jakes, Ross; Goedert, Michael; and Fink, Anthony L., "Biophysical Properties of The Synucleins and Their Propensities to Fibrillate: INHIBITION OF α-SYNUCLEIN ASSEMBLY BY β- AND γ-SYNUCLEINS" (2002). Molecular Medicine Faculty Publications. 686.
https://digitalcommons.usf.edu/mme_facpub/686
