Secondary Structure of The Homologous Proteins, A A-fetoprotein and Serum Albumin, from Their Circular Dichroism and Infrared Spectra

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Proteins Fetoprotein, Serum Albumin, Circular Dichroism, Human Serum Albumin (HSA)

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Secondary structure composition of two homologous proteins, alpha-fetoprotein and serum albumin, has been compared using circular dichroism and Fourier-transform infrared spectroscopy. CD-only analysis gives an accurate a-helical content for SA, but underestimates the predicted value for AFP. In contrast, FTIR-only analysis underestimates the helical content of SA. However, analysis of hybrid IR-CD spectra gives more accurate values for secondary structure composition for both AFP and SA and shows that the secondary structures of these proteins are the same.

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Citation / Publisher Attribution

Protein & Peptide Letters, v. 8, issue 4, p. 297-302