A New Scheme for the Isolation and Purification of Human Alpha-fetoprotein
A new efficient scheme for the isolation and purification of human alpha-fetoprotein from human cord serum was proposed. Sequential chromatography on four columns (Cibacron Blue Sepharose, two metal chelate, and one reversed-phase) helped rapidly prepare alpha-fetoprotein samples of high purity (no less than 98%; purification factor approximately 10(3)) in high yields (approximately 85%).
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Citation / Publisher Attribution
Bioorganicheskaia Khimiia, v. 25, issue 6, p. 412-417
Scholar Commons Citation
Uversky, Vladimir N. and Tomashevsky, Alu, "A New Scheme for the Isolation and Purification of Human Alpha-fetoprotein" (1999). Molecular Medicine Faculty Publications. 653.