A New Scheme for the Isolation and Purification of Human Alpha-fetoprotein

Authors

Vladimir N. Uversky, Institute for Biological Instrumentation of the Russian Academy of SciencesFollow
Alu Tomashevsky, Institute for Biological Instrumentation of the Russian Academy of Sciences

Document Type

Article

Publication Date

1999

Abstract

A new efficient scheme for the isolation and purification of human alpha-fetoprotein from human cord serum was proposed. Sequential chromatography on four columns (Cibacron Blue Sepharose, two metal chelate, and one reversed-phase) helped rapidly prepare alpha-fetoprotein samples of high purity (no less than 98%; purification factor approximately 10(3)) in high yields (approximately 85%).

Was this content written or created while at USF?

Yes

Citation / Publisher Attribution

Bioorganicheskaia Khimiia, v. 25, issue 6, p. 412-417

Scholar Commons Citation

Uversky, Vladimir N. and Tomashevsky, Alu, "A New Scheme for the Isolation and Purification of Human Alpha-fetoprotein" (1999). Molecular Medicine Faculty Publications. 653.
https://digitalcommons.usf.edu/mme_facpub/653