Ligand-free Form of Human α-fetoprotein: Evidence for the Molten Globule State
Digital Object Identifier (DOI)
By means of circular dichroism and fluorescence spectroscopy, viscometry and scanning microcalorimetry we have shown that the release of ligands from human α-fetoprotein (AFP) results in a considerable rearrangement of the protein molecule. Ligand-free form is practically as compact as the native molecule and has native-like content of secondary structure but no rigid tertiary structure. This means that the release of ligands transforms the AFP molecule into a molten globule state. Stripping the ligands from AFP is the irreversible process, i.e., native protein molecule cannot be reconstituted from the ligand-free form of AFP by adding back ligands. A possible functional role of such a structural transformation is discussed.
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Citation / Publisher Attribution
FEBS Letters, v. 410, issue 2-3, p. 280-284
Scholar Commons Citation
Uversky, Vladimir N.; Narizhneva, Natalya V.; Ivanova, Tatyana V.; Kirkitadze, Marina D.; and Tomashevsky, Andrey Yu., "Ligand-free Form of Human α-fetoprotein: Evidence for the Molten Globule State" (1997). Molecular Medicine Faculty Publications. 642.