Structural Properties of Ribosomal Protein S8 from the Extreme Thermophile Thermus Thermophilus

Authors

Valentina S. Vysotskaia
U F. Nasibullin
Vladimir N. Uversky, Institute of Protein Research of the Russian Academy of SciencesFollow
K S. Vasilenko, Institute of Protein Research of the Russian Academy of Sciences
Natalya V. Narizhneva, Institute of Protein Research of the Russian Academy of Sciences
Marina B. Garber, Institute of Protein Research of the Russian Academy of Sciences

Document Type

Article

Publication Date

1995

Abstract

The gene of ribosomal protein S8 from the extreme thermophile Thermus thermophilus was expressed in E. coli using the strain BL21(DE3) and vector pET3-1. A method of isolating this protein from the super producing strain was developed, which makes it possible to obtain 8-12 mg of product from 11 of culture. The secondary structure of protein S8 was determined by using CD spectroscopy. The protein was shown to be highly resistant to denaturants.

Was this content written or created while at USF?

Yes

Citation / Publisher Attribution

Bioorganicheskaia Khimiia, v. 21, issue 7, p. 492-497

Scholar Commons Citation

Vysotskaia, Valentina S.; Nasibullin, U F.; Uversky, Vladimir N.; Vasilenko, K S.; Narizhneva, Natalya V.; and Garber, Marina B., "Structural Properties of Ribosomal Protein S8 from the Extreme Thermophile Thermus Thermophilus" (1995). Molecular Medicine Faculty Publications. 630.
https://digitalcommons.usf.edu/mme_facpub/630