Title

‘All-or-none’ Mechanism of the Molten Globule Unfolding

Authors

Vladimir N. Uversky, Institute of Protein Research of the Russian Academy of SciencesFollow
G. V. Semisotnov, Institute of Protein Research of the Russian Academy of Sciences
R. H. Pain, University of Newcastle-upon-Tyne
O. B. Ptitsyn, Institute of Protein Research of the Russian Academy of Sciences

Document Type

Article

Publication Date

1992

Digital Object Identifier (DOI)

https://doi.org/10.1016/0014-5793(92)81468-2

Abstract

The Gdm-HCl-induced unfolding of bovine carbonic anhydrase B and S. aureus β-lactamase was studied at 4°C by a variety of methods. With the use of FPLC it has been shown that within the transition from the molten globule to the unfolded state the distribution function of molecular dimensions is bimodal. This means that equilibrium intermediates between the molten globule and the unfolded states are absent, i.e. the molten globule unfolding follows the ‘all-or-none’ mechanism.

Was this content written or created while at USF?

Yes

Citation / Publisher Attribution

FEBS Letters, v. 314, issue 1, p. 89-92

Scholar Commons Citation

Uversky, Vladimir N.; Semisotnov, G. V.; Pain, R. H.; and Ptitsyn, O. B., "‘All-or-none’ Mechanism of the Molten Globule Unfolding" (1992). Molecular Medicine Faculty Publications. 621.
https://digitalcommons.usf.edu/mme_facpub/621