‘All-or-none’ Mechanism of the Molten Globule Unfolding
Digital Object Identifier (DOI)
The Gdm-HCl-induced unfolding of bovine carbonic anhydrase B and S. aureus β-lactamase was studied at 4°C by a variety of methods. With the use of FPLC it has been shown that within the transition from the molten globule to the unfolded state the distribution function of molecular dimensions is bimodal. This means that equilibrium intermediates between the molten globule and the unfolded states are absent, i.e. the molten globule unfolding follows the ‘all-or-none’ mechanism.
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Citation / Publisher Attribution
FEBS Letters, v. 314, issue 1, p. 89-92
Scholar Commons Citation
Uversky, Vladimir N.; Semisotnov, G. V.; Pain, R. H.; and Ptitsyn, O. B., "‘All-or-none’ Mechanism of the Molten Globule Unfolding" (1992). Molecular Medicine Faculty Publications. 621.