‘All-or-none’ Mechanism of the Molten Globule Unfolding
Document Type
Article
Publication Date
1992
Digital Object Identifier (DOI)
https://doi.org/10.1016/0014-5793(92)81468-2
Abstract
The Gdm-HCl-induced unfolding of bovine carbonic anhydrase B and S. aureus β-lactamase was studied at 4°C by a variety of methods. With the use of FPLC it has been shown that within the transition from the molten globule to the unfolded state the distribution function of molecular dimensions is bimodal. This means that equilibrium intermediates between the molten globule and the unfolded states are absent, i.e. the molten globule unfolding follows the ‘all-or-none’ mechanism.
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
FEBS Letters, v. 314, issue 1, p. 89-92
Scholar Commons Citation
Uversky, Vladimir N.; Semisotnov, G. V.; Pain, R. H.; and Ptitsyn, O. B., "‘All-or-none’ Mechanism of the Molten Globule Unfolding" (1992). Molecular Medicine Faculty Publications. 621.
https://digitalcommons.usf.edu/mme_facpub/621
