Accelerated Fibrillation of α-synuclein Induced by The Combined Action of Macromolecular Crowding and Factors Inducing Partial Folding
Document Type
Article
Publication Date
2009
Keywords
Gags, Parkinson's Disease, Aggregation, Crowding, Fibrillation, Metals, Organic Solvent, Pesticides, Polycations, α-synuclein
Digital Object Identifier (DOI)
https://doi.org/10.2174/156720509788486491
Abstract
To better model the characteristics of crowded intracellular environments, we examined the effect of several factors known to induce partial folding and accelerated fibrillation of α-synuclein in dilute solutions, on the fibrillation of this protein in a crowded milieu. We found that low pH, certain metals and pesticides, polyanions, polycations and low concentrations of organic solvents cause a significant acceleration of α-synuclein fibrillation in the presence of high concentrations of polyethylene glycol. This suggests that the fibril-promoting effects of factors inducing partial folding of α- synuclein and the fibril-stimulating effects of macromolecular crowding are relatively independent and thus might act additively or even synergistically.
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
Current Alzheimer Research, v. 6, issue 3, p. 252-260
Scholar Commons Citation
Munishkina, Larissa A.; Fink, Anthony L.; and Uversky, Vladimir N., "Accelerated Fibrillation of α-synuclein Induced by The Combined Action of Macromolecular Crowding and Factors Inducing Partial Folding" (2009). Molecular Medicine Faculty Publications. 559.
https://digitalcommons.usf.edu/mme_facpub/559
