Influence of Serum Proteins on Conformation of Prostate-specific Antigen
Document Type
Article
Publication Date
2012
Digital Object Identifier (DOI)
https://doi.org/10.1080/073911012010525030
Abstract
Partition behavior of prostate-specific antigen (PSA) was studied in aqueous Dextran-Ficoll two-phase system. It was found that the partitioning of PSA changed in the presence of other proteins, in particular, bovine serum albumin, human serum albumin, human transferrin, and human gamma-globulin. The partition coefficient of PSA in mixtures with increasing amounts of these proteins decreased along the S-shaped curve and dropped to essentially the same value at the 104-105 protein: PSA molar ratio. Partition behavior of the above proteins was examined separately. Partition coefficient of a protein represents the protein solvent exposed residues; i.e., it reflects the 3D-structure of the protein in solution. Partition of binary protein mixtures reflects the interaction of the two proteins and therefore characterizes the PSA-induced conformational changes in a protein agent and the change in the PSA conformation induced by a protein agent. In other words, the protein effect on the partition behavior of free PSA may be explained by the effect of the non-specific PSA-protein interactions on PSA conformation. Formation of such PSA-protein encounter complexes was shown to be dominated by the electrostatic forces, since the efficiency of a given protein agent to induce changes in the partition behavior of PSA was proportional to its absolute mean net charge. Furthermore, in agreement with the earlier hypothesis that the protein segments with increased dynamic propensities (i.e., ‘discrete breathers’) can be important for conformational transitions accompanying binding processes, our analysis of intrinsically disordered regions (IDR) in all the proteins examined showed that the propensity for intrinsic disorder is related to the PSA partition-modulating capability of the protein.
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
Journal of Biomolecular Structure and Dynamics, v. 29, issue 5, p. 1051-1064
Scholar Commons Citation
Fedotoff, Olga; Mikheeva, Larissa M.; Chait, Arnon; Uversky, Vladimir N.; and Zaslavsky, Boris Y., "Influence of Serum Proteins on Conformation of Prostate-specific Antigen" (2012). Molecular Medicine Faculty Publications. 498.
https://digitalcommons.usf.edu/mme_facpub/498
