DRM1 and DRM2 Expression Regulation: Potential Role of Splice Variants in Response to Stress and Environmental Factors in Arabidopsis
Document Type
Article
Publication Date
2014
Keywords
DRM1, ARP, Dormancy-associated gene1, Auxin-repressed protein, RT qPCR, Intrinsically disordered protein, Splice variants, Light stress, Salt stress
Digital Object Identifier (DOI)
https://doi.org/10.1007/s00438-013-0804-2
Abstract
The DRM1/ARP gene family is increasingly exhibiting associations with stress conditions at the transcript level. Traditionally correlated with dormancy, increases in transcript levels in response to various treatments have also been reported in various species. As alternative transcript splicing is common in stress conditions, the splice variants of AtDRM1 and AtDRM2 were assessed further in this study. A previously undescribed splice variant of AtDRM1 (AtDRM1.6) is introduced in this work. In silico analyses of predicted protein sequence of all splice variants showed that all variants retain the predicted intrinsically disordered nature. Transcriptional studies of AtDRM1 and AtDRM2 in response to a wide range of abiotic, physical and hormonal treatments showed that AtDRM1.6 is differentially regulated at the transcriptional level compared with other splice variants. Promoter analyses demonstrated AtDRM1 light regulation via the upstream promoter sequence.
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
Molecular Genetics and Genomics, v. 289, issue 3, p. 317-332
Scholar Commons Citation
Rae, Georgina M.; Uversky, Vladimir N; David, Karine; and Wood, Marion, "DRM1 and DRM2 Expression Regulation: Potential Role of Splice Variants in Response to Stress and Environmental Factors in Arabidopsis" (2014). Molecular Medicine Faculty Publications. 429.
https://digitalcommons.usf.edu/mme_facpub/429
