The Use of Human, Bovine, and Camel Milk Albumins in Anticancer Complexes with Oleic Acid
Document Type
Article
Publication Date
2018
Keywords
Albumin, Anticancer, Apoptosis, Camel milk, Oleic acid
Digital Object Identifier (DOI)
https://doi.org/10.1007/s10930-018-9770-1
Abstract
Oleic acid (OA) is a monounsaturated fatty acid that upon binding to milk proteins, such as α-lactalbumin and lactoferrin, forms potent complexes, which exert selective anti-tumor activity against malignant cells but are nontoxic for healthy normal cells. We showed that the interaction of OA with albumins isolated from human, bovine, and camel milk results in the formation of complexes with high antitumor activity against Caco-2, HepG-2, PC-3, and MCF-7 tumor cells. The antitumor effect of the complexes is mostly due to the action of oleic acid, similar to the case of OA complexes with other proteins. Viability of tumor cells is inhibited by the albumin-OA complexes in a dose dependent manner, as evaluated by the MTT assay. Strong induction of apoptosis in tumor cells after their treatment with the complexes was monitored by flow cytometry, cell cycle analysis, nuclear staining, and DNA fragmentation methods. The complex of camel albumin with OA displayed the most pronounced anti-tumor effects in comparison with the complexes of OA with human and bovine albumins. Therefore, these results suggest that albumins have the potential to be used as efficient and low cost means of tumor treatment.
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
The Protein Journal, v. 37, p. 203-215
Scholar Commons Citation
El-Fakharany, Esmail M.; Abu-Serie, Marwa M.; Litus, Ekaterina A.; Permyakov, Sergei E.; Permyakov, Eugene A.; Uversky, Vladimir N.; and Redwan, Elrashdy M., "The Use of Human, Bovine, and Camel Milk Albumins in Anticancer Complexes with Oleic Acid" (2018). Molecular Medicine Faculty Publications. 241.
https://digitalcommons.usf.edu/mme_facpub/241
