Folding of Poly-amino Acids and Intrinsically Disordered Proteins in Overcrowded Milieu Induced by PH Change
Document Type
Article
Publication Date
2019
Digital Object Identifier (DOI)
https://doi.org/10.1016/j.ijbiomac.2018.12.038
Abstract
pH-induced structural changes of the synthetic homopolypeptides poly-E, poly-K, poly-R, and intrinsically disordered proteins (IDPs) prothymosin α (ProTα) and linker histone H1, in concentrated PEG solutions simulating macromolecular crowding conditions within the membrane-less organelles, were characterized. The conformational transitions of the studied poly-amino acids in the concentrated PEG solutions depend on the polymerization degree of these homopolypeptides, the size of their side chains, the charge distribution of the side chains, and the crowding agent concentration. The results obtained for poly-amino acids are valid for IDPs having a significant total charge. The overcrowded conditions promote a significant increase in the cooperativity of the pH-induced coil–α-helix transition of ProTα and provoke histone H1 aggregation. The most favorable conditions for the pH-induced structural transitions in concentrated PEG solutions are realized when the charged residues are grouped in blocks, and when the distance between the end of the side group carrying charge and the backbone is small. Therefore, the block-wise distribution of charged residues within the IDPs not only plays an important role in the liquid–liquid phase transitions, but may also define the expressivity of structural transitions of these proteins in the overcrowded conditions of the membrane-less organelles.
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
International Journal of Biological Macromolecules, v. 125, p. 244-255
Scholar Commons Citation
Fonin, Alexander V.; Stepanenko, Olga V.; Sitdikova, Asiia K.; Antifeeva, Iuliia A.; Kostyleva, Elena I.; Polyanichko, Alexander M.; Karasev, Maksim M.; Silonov, Sergey A.; Povarova, Olga I.; Kuznetsova, Irina M.; Uversky, Vladimir N.; and Turoverov, Konstantin К., "Folding of Poly-amino Acids and Intrinsically Disordered Proteins in Overcrowded Milieu Induced by PH Change" (2019). Molecular Medicine Faculty Publications. 132.
https://digitalcommons.usf.edu/mme_facpub/132
