Tudor Staphylococcal Nuclease is a Docking Platform for Stress Granule Components and is Essential for SnRK1 Activation in Arabidopsis

Authors

Emilio Gutierrez-Beltran, Universidad de Sevilla Sevilla Spain
Pernilla H. Elander, Uppsala BioCenter Swedish University of Agricultural Sciences and Linnean Center for Plant Biology
Kerstin Dalman, Uppsala BioCenter Swedish University of Agricultural Sciences and Linnean Center for Plant Biology
Guy W. Dayhoff, University of South Florida
Panagiotis N. Moschou, Institute of Molecular Biology and Biotechnology Foundation for Research and Technology - Hellas Heraklion Greece
Vladimir N. Uversky, University of South FloridaFollow
Jose L. Crespo, Universidad de Sevilla Sevilla Spain
Peter V. Bozhkov, Uppsala BioCenter Swedish University of Agricultural Sciences and Linnean Center for Plant Biology

Document Type

Article

Publication Date

2021

Keywords

heat stress, intrinsically disordered regions, SnRK1/SNF1/AMPK, stress granules, tudor staphylococcal nuclease

Digital Object Identifier (DOI)

https://doi.org/10.15252/embj.2020105043

Abstract

Tudor staphylococcal nuclease (TSN; also known as Tudor-SN, p100, or SND1) is a multifunctional, evolutionarily conserved regulator of gene expression, exhibiting cytoprotective activity in animals and plants and oncogenic activity in mammals. During stress, TSN stably associates with stress granules (SGs), in a poorly understood process. Here, we show that in the model plant Arabidopsis thaliana, TSN is an intrinsically disordered protein (IDP) acting as a scaffold for a large pool of other IDPs, enriched for conserved stress granule components as well as novel or plant-specific SG-localized proteins. While approximately 30% of TSN interactors are recruited to stress granules de novo upon stress perception, 70% form a protein–protein interaction network present before the onset of stress. Finally, we demonstrate that TSN and stress granule formation promote heat-induced activation of the evolutionarily conserved energy-sensing SNF1-related protein kinase 1 (SnRK1), the plant orthologue of mammalian AMP-activated protein kinase (AMPK). Our results establish TSN as a docking platform for stress granule proteins, with an important role in stress signalling.

Rights Information

This work is licensed under a Creative Commons Attribution 4.0 License.

Was this content written or created while at USF?

Yes

Citation / Publisher Attribution

The EMBO Journal, v. 40, issue 17, art. e105043

Scholar Commons Citation

Gutierrez-Beltran, Emilio; Elander, Pernilla H.; Dalman, Kerstin; Dayhoff, Guy W.; Moschou, Panagiotis N.; Uversky, Vladimir N.; Crespo, Jose L.; and Bozhkov, Peter V., "Tudor Staphylococcal Nuclease is a Docking Platform for Stress Granule Components and is Essential for SnRK1 Activation in Arabidopsis" (2021). Molecular Medicine Faculty Publications. 118.
https://digitalcommons.usf.edu/mme_facpub/118