Protein Flexibility and Cryoenzymology: the Trade-off Between Stability and Catalytic Rates
Document Type
Book Chapter
Publication Date
2023
Keywords
Enzyme, Enzymology, Cryoenzymology, Catalysis, Reaction Intermediate, ATP Synthase, Chymotrypsin, Cryo-em, Cryosolvents, Fast Reaction Kinetics, Glucosidases, Papain, Protein Folding, Psychrophilic Organisms
Digital Object Identifier (DOI)
https://doi.org/10.1016/B978-0-323-99533-7.00002-9
Abstract
Successful resolution of the specific details of the molecular mechanisms of catalytic reactions requires knowledge of the existence of intermediates on the reaction pathway and depends on the ability to structurally characterize such intermediates. The extremely high rates of the enzyme-catalyzed reactions and extremely short lifetimes of the reaction intermediates represent major bottlenecks in studying the dynamic processes taking place during catalysis. Overall, under the conditions typically used for the biocatalytic analysis, such rapidity precludes structural characterization of reaction intermediates by most high-resolution structural methods, as these intermediates are not accumulated in high enough concentrations and do not exist for long enough time periods. These limitations defined a strong desire for elaboration of experiential approaches that would permit detection and characterization of short-lived kinetic intermediates. Since many processes are dramatically slowed down at very low temperatures, cryoenzymology was considered a potential solution for the related problems, as the use of very low temperatures potentially allows the accumulation and stabilization of intermediates. This chapter describes some aspects pertaining to the use of cryoenzymology in the analysis of biological reactions catalyzed by proteins.
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
Protein Flexibility and Cryoenzymology: the Trade-off Between Stability and Catalytic Rates, in M. N. Gupta & V. N. Uversky (Eds.), Structure and Intrinsic Disorder in Enzymology, Academic Press, p. 73-95
Scholar Commons Citation
Gupta, Munishwar Nath and Uversky, Vladimir N., "Protein Flexibility and Cryoenzymology: the Trade-off Between Stability and Catalytic Rates" (2023). Molecular Medicine Faculty Publications. 1122.
https://digitalcommons.usf.edu/mme_facpub/1122
