Document Type
Article
Publication Date
2022
Keywords
L. Fermentum, Antibiotic Resistance, Prebiotics, Probiotics, Antibacterial Activity, Immunoregulation, S. Aureus, E. Coli, Salmonella, Campylobacter
Digital Object Identifier (DOI)
https://doi.org/10.3390/antibiotics11101437
Abstract
Limosilactobacillus fermentum strain 3872 (LF3872) was originally isolated from the breast milk of a healthy woman during lactation and the breastfeeding of a child. The high-quality genome sequencing of LF3872 was performed, and a gene encoding a unique bacteriocin was discovered. It was established that the bacteriocin produced by LF3872 (BLF3872) belongs to the family of cell-wall-degrading proteins that cause cell lysis. The antibacterial properties of LF3872 were studied using test cultures of antibiotic-resistant Gram-positive and Gram-negative pathogens. Gram-positive pathogens (Staphylococcus aureus strain 8325-4 and S. aureus strain IIE CI-SA 1246) were highly sensitive to the bacteriolytic action of LF3872. Gram-negative pathogens (Escherichia coli, Salmonella strains, and Campylobacter jejuni strains) were more resistant to the bacteriolytic action of LF3872 compared to Gram-positive pathogens. LF3872 is a strong co-aggregator of Gram-negative pathogens. The cell-free culture supernatant of LF3872 (CSLF3872) induced cell damage in the Gram-positive and Gram-negative test cultures and ATP leakage. In the in vitro experiments, it was found that LF3872 and Actigen prebiotic (Alltech Inc., Nicholasville, KY, USA) exhibited synergistic anti-adhesive activity against Gram-negative pathogens. LF3872 has immunoregulatory properties: it inhibited the lipopolysaccharide-induced production of proinflammatory cytokines IL-8, IL-1β, and TNF-α in a monolayer of Caco-2 cells; inhibited the production of IL-12 and stimulated the production of IL-10 in immature human dendritic cells; and stimulated the production of TGF-β, IFN-γ, and IgA in the immunocompetent cells of intestinal Peyer’s patches (PPs) in mice. These results indicate the possibility of creating a synbiotic based on LF3872 and a prebiotic derived from Saccharomyces cerevisiae cell wall components. Such innovative drugs and biologically active additives are necessary for the implementation of a strategy to reduce the spread of antibiotic-resistant strains of socially significant animal and human infections.
Rights Information
This work is licensed under a Creative Commons Attribution 4.0 License.
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
Antibiotics, v. 11, issue 10, art. 1437
Scholar Commons Citation
Abramov, Vyacheslav M.; Kosarev, Igor V.; Machulin, Andrey V.; Priputnevich, Tatiana V.; Chikileva, Irina O.; Deryusheva, Eugenia I.; Abashina, Tatiana N.; Donetskova, Almira D.; Panin, Alexander N.; Melnikov, Vyacheslav G.; Suzina, Natalia E.; Nikonov, Ilya N.; Selina, Marina V.; Khlebnikov, Valentin S.; Sakulin, Vadim K.; Vasilenko, Raisa N.; Samoilenko, Vladimir A.; Uversky, Vladimir N.; and Karlyshev, Andrey V., "Limosilactobacillus fermentum Strain 3872: Antibacterial and Immunoregulatory Properties and Synergy with Prebiotics Against Socially Significant Antibiotic-resistant Infections of Animals and Humans" (2022). Molecular Medicine Faculty Publications. 1004.
https://digitalcommons.usf.edu/mme_facpub/1004
