Abstract
This dataset contains concentrations of Tri-iodothyronine (T3 ), Thyroxine (T4), and Thyroid Stimulating Hormone (TSH) in adult pompano after exposure to a chemically enhanced water-accommodated fraction (CEWAF) of south Louisiana crude oil and reproduction.
Purpose
Identify changes in thyroid function in adult pompano after exposure to CEWAF of south Louisiana crude oil and reproduction.
Keywords
Thyroid, Reproduction, Tri-iodothyronine (T3), Thyroxine (T4), Thyroid Stimulating Hormone (TSH), Florida Pompano, Trachinotus carolinus, Chemically enhanced water accommodated fraction (CEWAF), Crude oil
UDI
R6.x805.000:0069
Date
April 2020
Point of Contact
Name
Dana Wetzel
Organization
Mote Marine Laboratory / Environmental Laboratory of Forensics Program
Name
Rebecca Medvecky
Organization
Mote Marine Laboratory / Environmental Laboratory of Forensics Program
Funding Source
RFP-6
DOI
10.7266/n7-3nty-7169
Rights Information
This work is licensed under a
Creative Commons Public Domain Dedication 1.0 License.
Scholar Commons Citation
Dana Wetzel. 2020. Chemically enhanced water-accommodated fractions (CEWAF)- exposed Florida pompano reproduction: Thyroid Function Assays. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-3nty-7169
Comments
Extent
The dataset contains laboratory measurements of reproduction, no field sampling was involved.
Supplemental Information
Dataset consists of 1 Excel file with multiple worksheets that detail the experimental setup and conditions (Naming Convention, T208 Sample Key, Test Conditions Table, Tank Descriptions, and ELISA). The worksheet "Naming Convention": Sample IDs are named as "CII-15-0000", where three letter laboratory project code (CII=CIMAGE-II)-two digit year (2015=15)-four digit integral assigned by laboratory beginning at 0000 for each new year(0000 through 9999). The worksheet “T208 Sample Key” includes - Collected Date (MM/DD/YYYY), Field ID (Fish #), Matrix (Plasma-EDTA), MML Number, Species (Florida Pompano), Gender (Male/Female), Weight (g), and Length (cm). The worksheet “Tank Descriptions” includes - Tank, # Female, # Male, Treatment (Control/CEWAF), Start Date (MM/DD/YYYY), End Date (MM/DD/YYYY), Date Spike A, Time Spike A, Date Spike B, Time Spike B, and Notes. The worksheets “ELISA” includes - Spawning tank, Goiter, Treatment (CEWAF/Control), Fish ID, and replicate measurements of Cusabio T4 (ng/ml), Cusabio TSH (Ug/ml), and Cusabio T3 (ng/ml). The worksheet “Test Conditions Table” includes - Test Name (Pompano Adult Water Exposure), Test ID: 208, Species tested (Florida Pompano), Life-stage/age (Adult), Test Duration, Exposure Type, Renewal frequency, Number of replicates per treatment (9), Organisms per tank, Temperature, Salinity, Number of Treatments, and the description of treatments.|Plasma samples were analyzed using ELISA kits prepared by Cusabio for T3 (CSB E084888), T4 (CSB E08489F), and TSH (CSB EQ027261FI) according to manufacturer protocols.||||