Abstract
This dataset contains total polycyclic aromatic hydrocarbon (TPAH) concentrations in exposure seawater solutions and in livers of laboratory-raised Florida pompano exposed to south Louisiana crude oil.
Purpose
To evaluate the TPAH concentrations of south Louisiana crude oil in exposure seawater and in the tissue of laboratory-raised Florida pompano exposed to south Louisiana crude oil.
Keywords
Deepwater Horizon, Florida Pompano, CEWAF, TPAH, total polycyclic aromatic hydrocarbon, chemically enhanced water-accommodated fractions
UDI
R6.x805.000:0070
Date
November 2019
Point of Contact
Name
Dana Wetzel
Organization
Mote Marine Laboratory / Environmental Laboratory of Forensics Program
Name
Rebecca Medvecky
Organization
Mote Marine Laboratory / Environmental Laboratory of Forensics Program
Funding Source
RFP-6
DOI
10.7266/n7-97xn-0v49
Rights Information
This work is licensed under a
Creative Commons Public Domain Dedication 1.0 License.
Scholar Commons Citation
Wetzel, Dana; Sherwood, Tracy; Medvecky, Rebecca; Miller, Christelle. 2019. Total polycyclic aromatic hydrocarbon (TPAH) results: Chemically enhanced water-accommodated fractions (CEWAF)-exposed Florida pompano F1 Generation. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-97xn-0v49
Comments
Extent
Dataset contains laboratory measurements of oil in water and tissue samples. All fish were bred and raised at Mote Aquaculture Research Park, no field sampling was involved.
Supplemental Information
Sample Key: MML; Field ID; Matrix; Collected Date; Collected Time; Species; Organism Weight(g); Fork Length (cm); Comment Stock CEWAF Preparation: Seawater (L); End Date; End Stir Time; Stir Speed (rpm); Settle Time (hr); Notes; Prepared by CEWAF Dilution Preparation: Date; Time; Carboy ID; Stock Vol (L); Total Stock (L); Final Vol (L); Prepared By Tank Description: Tank; Treatment; Start Date; End Date; Date Spike A; Time Spike A; Time Clean SW A; Date Spike B; Time Spike B; Time Clean SW B; Notes Water TPAH: Field ID; MML; Date; Analytes; MDL (ug/L); ΣPAHs(ug/L) Liver ΣPAH_WW: Field ID; MML; Parent Cross PAH Analytes; MDL (ng/g ww); ΣPAH (ng/g ww) % Lipid: MML; Field ID; % Lipid Note: MML= Mote Marine Laboratory and MDL= Minimum Detectable Limit Nomenclature for sample IDs example: "CII-15-0000" means "Three-letter laboratory project code (CII=CIMAGE-II)-two digit year(2015=15)-four digit integral assigned by laboratory beginning at 0000 for each new year(0000 through 9999)".|Tissue samples were surrogated with naphthalene d8, acenaphthene d10, phenanthrene-d10, chrysene-d12, perylene d12 and extracted via EPA method 3545 using a Dionex ASE300, then concentrated to 10ml in DCM for analysis. Water samples were surrogated with naphthalene d8, acenaphthene d10, phenanthrene-d10, chrysene-d12, perylene d12 and extracted via EPA method 3510, and concentrated to 1mL in DCM for analysis. PAHs were analyzed using gas chromatography/mass spectrometry in selected ion monitoring mode using a 0.25 um film thickness, 0.25 mm I.D., and 30 m length capillary column. One microliter of the sample was injected at 60ºC, held for 1.5 minutes. The temperature was then increased to 325 ºC at a rate of 8 ºC/ min and held for 3 min.|Dionex ASE300 Accelerated Solvent Extractor, Agilent 7890A/5975C, Zebron Capillary column.||Average surrogate recovery 50-150. CCV run before and after every ten samples. CCV passing recovery must be average less than 20%. CCV means Continued Calibration Verification.|