Abstract
Sediment samples collected from the northern Gulf of Mexico were used for incubations in which microbial respiration rates were measured as a proxy for biodegradation of hydrocarbons. Following the incubation, microbial communities were characterized from incubation samples using next generation sequencing of SSU rRNA genes. A systematic approach was used whereby pressure, temperature, and hydrocarbon substrate were varied.
Purpose
The purpose of the dataset was to compare the impact of hydrostatic pressure on biodegradation of crude oil.
Keywords
hydrocarbon degradation, microbial community, pressure effect, microbial respiration
UDI
R6.x805.000:0057
Date
December 2020
Point of Contact
Name
Joel E. Kostka
Organization
Georgia Institute of Technology / School of Earth and Atmospheric Sciences
Name
Xiaoxu Sun
Organization
Georgia Institute of Technology / School of Earth and Atmospheric Sciences
Funding Source
RFP-6
DOI
10.7266/n7-eg4t-1g22
Rights Information
This work is licensed under a
Creative Commons Public Domain Dedication 1.0 License.
Scholar Commons Citation
Sun, Xiaoxu. 2020. Impacts of high pressure on hydrocarbon biodegradation. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-eg4t-1g22
Comments
Extent
Dataset contains laboratory measurements, no field sampling involved.
Supplemental Information
Sample equipment: multicore; Excursion or Cruise ID: WB0815; Collection date month/day/year: 09/08/2016; Decimal latitude and longitude: 28 58.946N 87 53.665W; Depth of water in meters: 1468; Depth of sediment in centimeters 0-5; Respiration rates by treatment; Abundance, diversity and composition of microbial communities based on SSU rRNA gene sequences; Sample equipment: multicore; Excursion or Cruise ID: WB0815; Collection date month/day/year: 09/09/2016; Decimal latitude and longitude: 29 07.444N -87 15.94W; Depth of water in meters: 924; Depth of sediment in centimeters: 0-5; Respiration rates by treatment; Abundance, diversity and composition of microbial communities based on SSU rRNA gene sequences; Sample equipment: multicore; Excursion or Cruise ID: WB0815; Collection date month/day/year: 08/11/2016; Decimal latitude and longitude: 18 59.149 92 09.080; Depth of water in meters:19; Depth of sediment in centimeters 0-5; Respiration rates by treatment; Abundance, diversity and composition of microbial communities based on SSU rRNA gene sequences|The samples were collected using multicore. The retrieved samples were extruded based on depth on board. Samples were kept at 4 degrees Celsius until further processing. The preparation of samples for incubations were conducted in a cold room at 4C the whole time for maintaining the temperature. Surface sediment samples were mixed with filtered seawater from the same site. 1ul crude oil was provided. Samples were incubated in heat-sealable polypropylene tubes in pressure-maintaining cells. Oxygen was measured as a proxy for oil degradation, and no oil control/ sterile controls were prepared as the background. DNA was extracted at different time points and sequenced for the SSU rRNA on an Illumina MiSeq.|Illumina MiSeq|||