Abstract
Controlled oil exposure study to evaluate the effects of south Louisiana crude oil chemically enhanced water accommodated fraction (CEWAF) exposure on Florida pompano and reproduction. Data include total polycyclic aromatic hydrocarbon (tPAH) results for exposure solutions, pompano livers, and pompano whole bodies, as well as volatile organic compound (VOC) results for exposure solutions.
Purpose
Controlled oil exposure study to evaluate the effects of south Louisiana crude oil CEWAF exposure on fish health and reproduction.
Keywords
PAH, Deepwater Horizon, CEWAF, Reproduction, Florida pompano, Trachinotus carolinus
UDI
R4.x267.000:0073
Date
January 2019
Point of Contact
Name
Dana Wetzel
Organization
Mote Marine Laboratory / Environmental Laboratory of Forensics Program
Funding Source
RFP-4
DOI
10.7266/N7CF9NMN
Rights Information
This work is licensed under a
Creative Commons Public Domain Dedication 1.0 License.
Scholar Commons Citation
DL Wetzel, TA Sherwood, CA Miller, RL Medvecky. 2019. CEWAF-exposed Florida pompano reproduction: tPAH results. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7CF9NMN
Comments
Extent
Dataset contains laboratory measurements of oil exposure on pompano tissues, no field sampling involved.
Supplemental Information
Tank Descriptions worksheet: Tank, # Female, # Male, Treatment, Start and End Dates (MM/DD/YYYY), Date Spike A (MM/DD/YYYY), Time Spike A, Date Spike B (MM/DD/YYYY), Time Spike B. 208 Sample Key worksheet: Collected date (MM/DD/YYYY), Collected Location (MAP - Mote Aquaculture Park), Field ID, Matrix, MML #, Species. Stock CEWAF Preparation worksheet: Date (MM/DD/YYYY), Start Time, Carboy ID, Oil (g), Corexit (g), Seawater (L). CEWAF Dilution Preparation worksheet: Date (MM/DD/YYYY), Time, Carboy ID, Stock Vol (L), Final Vol (L). TPH_TANK H2O_Results worksheet: Sample, Tank, TPH (ug/L), Date (MM/DD/YYYY). PAH_Tank_H2O Results worksheet: PAH Analyte, PAH units (ug/L), MDL (ug/L), MML, Treatment, Spike #, Tank, Sample Date (MM/DD/YYYY). Liver PAH Results worksheet: PAH Analyte, PAH units (ug/L), MDL (ug/L), MML, Treatment, Fish ID, Comment, Sample Date (MM/DD/YYYY). WHOLE BODY PAH Results worksheet: PAH Analyte, PAH units (ug/L), MDL (ug/L), MML, Treatment, Fish ID, Comment, Sample Date (MM/DD/YYYY). VOC Results worksheet: Analyte, Concentration (ug/L), Qualifier.|Tissue samples were surrogated with naphthalene d8, acenaphthene d10, phenanthrene-d10, chrysene-d12, perylene d12 and extracted via EPA method 3545 using a Dionex ASE300, then concentrated to 10ml in DCM for analysis. Water samples were surrogated with naphthalene d8, acenaphthene d10, phenanthrene-d10, chrysene-d12, perylene d12 and extracted via EPA method 3510, and concentrated to 1mL in DCM for analysis. PAHs were analyzed using gas chromatography/mass spectrometry in selected ion monitoring mode using a 0.25 um film thickness, 0.25 mm I.D., and 30 m length capillary column. One microliter of sample was injected at 60ºC, held for 1.5 minute. Temperature was then increased to 325 ºC at a rate of 8 ºC/ min, and held for 3 min. VOC samples were collected in accordance with Pace Analytical collection protocols, then sent to Pace Analytical for evaluation.|Dionex ASE300 Accelerated Solvent Extractor, Agilent 7890A/5975C, Zebron Capillary column||Average surrogate recovery 50-150. CCV run before and after every ten sample. CCV passing recovery must be average less than 20%.|