Abstract
This dataset contains results of lipid class composition using Iatroscan and fatty acid identification of Florida pompano exposed to dispersed south Louisiana crude oil. Florida pompano were either exposed to 1 or 0.5 ppm of chemically enhanced water accommodated fraction (CEWAF). A control experiment was also performed. After a specified period fish were dissected and lipids were extracted from liver tissue and whole body tissue and were analyzed.
Purpose
This is part of an experiment to see the effects of dispersed crude oil, via south Louisiana crude oil CEWAF, on the lipid content of fish.
Keywords
lipid composition, Florida pompano, Trachinotus carolinus, CEWAF, Iatroscan MK-6s
UDI
R4.x267.000:0059
Date
February 2019
Point of Contact
Name
Dana Wetzel
Organization
Mote Marine Laboratory / Environmental Laboratory of Forensics Program
Funding Source
RFP-4
DOI
10.7266/n7-m5b4-9956
Rights Information
This work is licensed under a
Creative Commons Public Domain Dedication 1.0 License.
Scholar Commons Citation
Dana Wetzel. 2019. CEWAF-exposed Florida pompano reproduction: Lipid Class Composition. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-m5b4-9956
Comments
Extent
Dataset contains laboratory measurements of lipids, no field sampling involved.
Supplemental Information
Sample key worksheet: MML, Matrix (Liver, Whole Body), Tag ID, Collected date (dd-mon-yy), Organism Species (Florida Pompano), Treatment (Control, Low, High), Spawning Tank. Exposure tank description and spawning tank description worksheets: Tank ID (A, B, C, D, E, F, G, H, I), Start date (mm/dd/yyyy), End date (mm/dd/yyyy), Treatment (High CEWAF, Low CEWAF, Control), Number of Fish (5), Notes, Recorded by (RM). CEWAF Prep worksheet: Start Date (mm/dd/yyyy), End Date (mm/dd/yyyy), Carboy (A, B, F), Oil (g), Corexit (g), Volume Water (L), Treatment (High, Low), Amount of Stock Solution (L), Amount of Seawater (L). % Lipid worksheet: Dissection Date (dd-mon-yy), Exposure Tank ID (Control, Low, High), Tag number, Liver, Lipid (%), Whole Body, Lipid (%). Liver TLC results and whole body TLC results worksheets: Treatment (Control, Low, High), Tag number, Rep, MML, Wax esters & Sterol Esters (%), Triglycerides (%), Free Fatty Acids (%), Cholesterol (%), Phospholipids & Polar Lipids (%).|Methods adapted from the NOAA technical memorandum Sloan et al., 2014.|Mitsubishi Kagaku Iatron, Inc., Iatroscan MK-6s, PeakSimple Chromatography Data System.|||Sloan, C. A., B. F. Anulacion, K. A. Baugh, J. L. Bolton, D. Boyd, R. H. Boyer, D. G. Burrows, D. P. Herman, R. W. Pearce, G. M. Ylitalo. (NWFSC) 2014. Northwest Fisheries Science Center's analyses of tissue, sediment, and water samples for organic contaminants by gas chromatography/mass spectrometry and analyses of tissue for lipid classes by thin layer chromatography/flame ionization detection. U.S. Dept. of Commerce, NOAA Tech. Memo., NMFS-NWFSC-125, 61 p.