Abstract
Cytokine concentrations in red drum after exposure to chemically enhanced water accommodated fraction (CEWAF) of south Louisiana crude oil. Ct and percent efficiencies of the deferentially expressed genes of red drum spleen, liver and gill tissue exposed to south Louisiana crude oil CEWAF are provided.
Purpose
To determine the effects of CEWAF exposure on red drum.
Keywords
red drum, cytokines, gene expression, Sciaenops ocellatus
UDI
R4.x267.000:0065
Date
February 2019
Point of Contact
Name
Dana Wetzel
Organization
Mote Marine Laboratory / Environmental Laboratory of Forensics Program
Funding Source
RFP-4
DOI
10.7266/n7-5hb7-zg41
Rights Information
This work is licensed under a
Creative Commons Public Domain Dedication 1.0 License.
Scholar Commons Citation
Dana Wetzel. 2019. Red drum CEWAF uptake/depuration study: Immune Function Assays & Gene Expression. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/n7-5hb7-zg41
Comments
Extent
Dataset contains laboratory measurements of cytokine concentration and gene expression, no field sampling involved.
Supplemental Information
Tank Descriptions worksheet: Tank ID, Start and End Date (MM/DD/YYYY), Treatment, Number of Fish, Notes, Recorded by. CEWAF Prep worksheet: Date (MM/DD/YYYY), Carboy, Oil (g), Corexit (g), Volume Water (L). Test 113 Sample Key worksheet: Day, Sample Date (MM/DD/YYYY), Sample Location (MAP - Mote Aquaculture Park), Tank ID (numbers in fron of Tank indicate tagged fish), Matrix, MML #, Fish ID, Treatment. ELISA worksheet: Whole Body MML, Whole Body tPAH (ng/g), Liver MML, Liver tPAH (ng/g), Fish ID, Day, Tank, Treatment. MML, rep 1, rep2, avg, stdev, %cv for lgM, IL-1B Abbexa, IL-8 MBS (for two dates), TNFa MBS (for two dates), IL-IB MBS (for two dates), MBS CXCL10. Units in pg/ml. Spleen ct worksheet: Matrix, Rep, MML, Treatment, Day, BA (Ct) House keeping gene, CXC10, fucolectin, GST (0.9), GST (1.5), CYP1A, UDP60, CYP26C1, GS. spleen efficiencies worksheet: Matrix, Rep, MML, Treatment, Day, BA (Ct) House keeping gene, CXC10, fucolectin, GST (0.9), GST (1.5), CYP1A, UDP60, CYP26C1, GS. Liver Ct worksheet: Matrix, Rep, MML, Treatment, Day, BA (Ct) House keeping gene, CXC10, CCL20, C4, fucolectin, GST (0.9), GST (1.5), CYP1A, UDP60, CYP26C1, GS. Liver efficiencies worksheet: Matrix, Rep, MML, Treatment, Day, BA (Ct) House keeping gene, CXC10, CCL20, C4, fucolectin, GST (0.9), GST (1.5), CYP1A, UDP60, CYP26C1, GS. Gill Ct worksheet: Matrix, Rep, MML, Treatment, Day, BA (Ct) House keeping gene, fucolectin, GST (0.9), CYP1A. Gill efficiencies worksheet: Matrix, Rep, MML, Treatment, Day, BA (Ct) House keeping gene, fucolectin, GST (0.9), CYP1A.|||||