Abstract
Lipid class composition of red drum fed a diet of pellets saturated with south Louisiana crude oil using Iatroscan.
Purpose
Controlled oil exposure study to evaluate the effects of crude oil on lipid structure in whole body of fish.
Keywords
Diet exposure, Deepwater Horizon, crude oil, lipid, phospholipid, red drum, Sciaenops ocellatus
UDI
R4.x267.000:0054
Date
December 2018
Point of Contact
Name
Dana Wetzel
Organization
Mote Marine Laboratory / Environmental Laboratory of Forensics Program
Funding Source
RFP-4
DOI
10.7266/N7TT4PFR
Rights Information
This work is licensed under a
Creative Commons Public Domain Dedication 1.0 License.
Scholar Commons Citation
DL Wetzel, TA Sherwood, CA Miller, RL Medvecky. 2018. Red drum diet exposure to Deepwater Horizon crude oil contaminated feed: Lipid Composition. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7TT4PFR
Comments
Extent
Dataset contains laboratory measurements of lipid composition, no field sampling involved.
Supplemental Information
Test 103 Sample Key worksheet: Date Collected (MM/DD/YYYY), Location Collected (MAP - Mote Aquaculture Park), Field ID, Matrix, MML, Species, Project, Tank ID, Treatment (High, Low, Control). Food Preparation worksheet: Prep start and end date, est. total mass needed for daily feeding and samples (g), feed prep. method, feed mass (g), mass of oil added (g), mixing speed, mixing time, oil source. % Lipid worksheet: FISH ID, Tank ID, Whole Body (sample ID), % Lipid, Liver (sample ID), % Lipid. Liver TLC Results worksheet: Fish ID, Tank ID, REP (replicate), Liver MML #, % Wax esters & Sterol Esters, % Triglycerides, % Free Fatty Acids, % Cholesterol, % Phospholipids & Polar Lipids. Whole Body TLC Results worksheet: Fish ID, Tank ID, REP (replicate), Liver MML #, % Wax esters & Sterol Esters, % Triglycerides, % Free Fatty Acids, % Cholesterol, % Phospholipids & Polar Lipids.|Tissue samples extracted via EPA method 3545 using a Dionex ASE300, then concentrated to 10 mL in DCM for analysis. Lipid Composition: 1 uL of sample analyzed with Iatroscan using PeakSimple 444 software.|Iatroscan MK-6s|NA|QA measures were included into per batch according to Environmental Laboratory for Forensics Quality Assurance Plan. If QA measurements were not met, samples were re-analyzed or data was qualified.|NA