Abstract
Lipid class composition of red drum exposed to south Louisiana crude oil CEWAF using Iatroscan.
Purpose
Controlled oil exposure study to evaluate the effects of crude oil on lipid structure in the whole body of fish.
Keywords
Uptake, Depuration, Deepwater Horizon, crude oil, Red drum, Sciaenops ocellatus
UDI
R4.x267.000:0063
Date
January 2019
Point of Contact
Name
Dana Wetzel
Organization
Mote Marine Laboratory / Environmental Laboratory of Forensics Program
Funding Source
RFP-4
DOI
10.7266/N7NZ8648
Rights Information
This work is licensed under a
Creative Commons Public Domain Dedication 1.0 License.
Scholar Commons Citation
DL Wetzel, TA Sherwood, CA Miller, RL Medvecky. 2019. Red drum CEWAF uptake/depuration study: Lipid Composition. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7NZ8648
Comments
Extent
The dataset contains laboratory measurements of lipid composition, no field sampling involved.
Supplemental Information
Test 113 Sample Key worksheet: Day, Sample Date (mm/dd/yyyy), Sample Location (MAP: Mote Aquaculture Park), Tank ID, Matrix, MML, Comments. Tank Descriptions worksheet: Tank ID, Start and End Date (mm/dd/yyyy), Treatment, Number of Fish. CEWAF Prep worksheet: Date (mm/dd/yyyy), Carboy, Oil (g), Corexit (g), Volume Water (L). Lipid Weight worksheet: Liver and Whole Body: g/10mL and % Lipid. TLC Liver Results and Whole Body Results worksheet: Day, Tank, Rep, MML #, % Wax esters & Sterol Esters, % Triglycerides, % Free Fatty Acids, % Cholesterol, % Phospholipids & Polar Lipids, % Total Lipid. For liver results: Dilutions indicated by d# (i.e d2=2x, d5=5x, d10=10x, d50=50x).|Tissue samples were extracted via EPA method 3545 using a Dionex ASE300, then concentrated to 10 mL in DCM for analysis. Lipid Composition: 1 uL of sample analyzed with Iatroscan using PeakSimple 444 software.|Iatroscan MK-6s||QA measures were included per batch according to Environmental Laboratory for Forensics Quality Assurance Plan. If QA measurements were not met, samples were re-analyzed or data was qualified.|