Abstract
Lipid class composition by Iatroscan of adult Florida pompano exposed to south Louisiana crude oil via CEWAF-spiked water.
Purpose
Controlled oil exposure study to evaluate the effects of crude oil on lipid structure in whole body of fish.
Keywords
Reproduction, Deepwater Horizon, crude oil, CEWAF, Florida pompano, Trachinotus carolinus
UDI
R4.x267.000:0074
Date
January 2019
Point of Contact
Name
Dana Wetzel
Organization
Mote Marine Laboratory / Environmental Laboratory of Forensics Program
Funding Source
RFP-4
DOI
10.7266/N77S7M7R
Rights Information
This work is licensed under a
Creative Commons Public Domain Dedication 1.0 License.
Scholar Commons Citation
DL Wetzel, TA Sherwood, CA Miller, RL Medvecky. 2019. CEWAF water exposed Florida pompano F0 reproduction: Lipid Class Composition. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N77S7M7R
Comments
Extent
Dataset contains laboratory measurements of lipid composition, no field sampling involved.
Supplemental Information
T208 Sample Key worksheet: Collected date (MM/DD/YYYY), Field ID, Treatment, Spawning Tank, Matrix, MML Number, Species, Gender, Weight (g), Length (cm). Tank Descriptions worksheet: Tank, # Female, # Male, Treatment, Start and End Date (MM/DD/YYYY), Date Spike A (MM/DD/YYYY), Time Spike A, Date Spike B (MM/DD/YYYY), Time Spike B. CEWAF Dilution Preparation worksheet: Date (MM/DD/YYYY), Time, Carboy ID, Stock Vol (L), Final Vol (L). % Lipid worksheet: Treatment, Fish, MML Liver, Liver % Lipid, MML Whole Body, Whole Body % Lipid. Liver TLC Results worksheet: Treatment, Fish ID, Rep, Liver MML #, % Wax esters and Sterol esters, % Triglycerides, % Free fatty acids, % Cholesterol, % Phospholipids and Polar lipids. Whole Body TLC Results worksheet: Treatment, Fish ID, Rep, Whole Fish MML #, % Wax esters and sterol esters, % Triglycerides, % Free fatty acids, % Cholesterol, % Phospholipids and Polar lipids.|Tissue samples were extracted via EPA method 3545 using a Dionex ASE300, then concentrated to 10 mL in DCM for analysis. Lipid Composition: 1 uL of sample analyzed with Iatroscan using PeakSimple 444 software.|Iatroscan MK-6s||QA measures were included into per batch according to Environmental Laboratory for Forensics Quality Assurance Plan. If QA measurements were not met, samples were re-analyzed or data was qualified.|