Dataset Authors

Shokouh Rahsepar

Abstract

This dataset contains experimental data on the biodegradation of oil in the sediment and effect of marine snow on oil biodegradation (oxygen consumption and n-alkane biodegradation).

Comments

Extent

Dataset contains laboratory measurements of oil degradation, no field sampling involved

Supplemental Information

The following is a list of data parameters and units per tab within the data file: Con. 1, GC data [n-alkanes (C9-C35) using C30-hopane (17a(H),21b(H)-hopane) normalized areas]: 1At1, 1Bt1, 1Ct1: 1 (refers to condition number), A (refers to replicates, A, B and C), t1 (refers to time (day), day 1st of incubation). Avg: refers to average of the three replicates (A, B and C). stdev: refers to standard deviation between three replicates; C9 - C35 – normalized area per n-alkane; Con. 3, GC data [n-alkanes (C9-C35) using C30-hopane (17a(H),21b(H)-hopane) normalized areas]: 1At1, 1Bt1, 1Ct1: 1 (refers to condition number), A (refers to replicates, A, B and C), t1 (refers to time (day), eg. day 1st of incubation). Avg: refers to average of the three replicates (A, B and C). stdev: refers to standard deviation between three replicates; C9 - C35 – normalized area per n-alkane; Temperature [Temperature (degree Celsius) in the water sample of aquaria]: Conditions – (Condition 1: “Snow + Oil”, Condition 2: “Snow”, Condition 3: “Oil”, Condition 4: “Clay control”, Condition 5: “Sediment control”); Replicates – (a, b, c); Day 0, 1, 3, 6, 9, 16, 21, 23, 29, 36, 42 – temperature of water sample per condition/replicate (C); Salinity [Salinity (‰) in the water sample of aquaria]: Conditions – (Condition 1: “Snow + Oil”, Condition 2: “Snow”, Condition 3: “Oil”, Condition 4: “Clay control”, Condition 5: “Sediment control”); Replicates – (a, b, c); Day 0, 3, 6, 9, 16, 21, 23, 29, 36, 42 – salinity of water sample per condition/replicate (‰); Oxygen% [Oxygen (% saturation) in the water sample of aquaria]: Conditions – (Condition 1: “Snow + Oil”, Condition 2: “Snow”, Condition 3: “Oil”, Condition 4: “Clay control”, Condition 5: “Sediment control”); Replicates – (a, b, c); Day 0, 1, 3, 6, 9, 16, 21, 23, 29, 36, 42 – Oxygen saturation of water sample per condition/replicate (%); Oxygen mgl [Oxygen (mg/L) in the water sample of aquaria]: Conditions – (Condition 1: “Snow + Oil”, Condition 2: “Snow”, Condition 3: “Oil”, Condition 4: “Clay control”, Condition 5: “Sediment control”); Replicates – (a, b, c); Day 0, 1, 3, 6, 9, 16, 21, 23, 29, 36, 42 – Oxygen in water sample per condition/replicate (mg/L); pH [pH (-) in the water sample of aquaria]: Conditions – (Condition 1: “Snow + Oil”, Condition 2: “Snow”, Condition 3: “Oil”, Condition 4: “Clay control”, Condition 5: “Sediment control”); Replicates – (a, b, c); Day 0, 1, 3, 6, 9, 16, 21, 23, 29, 36, 42 – pH in water sample per condition/replicate (-); The following is information describing the treatments used in the analyses: Sediment: natural uncontaminated sediment (≈ 3,2 L per each aquaria). Uncontaminated sediments were collected from the top 10 cm of an intertidal mudflat in the Dutch Wadden Sea (N 52° 56.112 E 004° 59.976) at low tide. Collected sediments were transported to the laboratory and sieved over a 1 mm sieve to remove larger organisms and particles. Oil: Surrogate Macondo oil, kindly provided by BP. 0.63 g of oil per aquaria. Clay: kaolin clay (hydrated aluminum silicate, CAS 1332-58-7, Sigma Aldrich). 3.15 g kaolin clay per aquaria Snow: Artificial marine snow was prepared in our laboratory by adding 22.5 g of commercially available alginate (alginic acid sodium salt, CAS 9005-38-3; 7.5 g for each aquarium replicate), to 1.1 L of filtered natural seawater while stirring. Then, 9.45 g of kaolin clay (hydrated aluminum silicate, CAS 1332-58-7, Sigma Aldrich; 3.15 g for each aquarium replicate) and 19 g of fresh weight of phytoplankton biomass (Chlorella pasta, Ingepro BV, Borculo, The Netherlands; 6.3 g for each aquarium replicate) were added to the solution. Separately, 188 g of CaCl2 (CAS 10043-52-4, Fluka Analytical) was dissolved in 13 L of demi-water and this solution was added to the alginate solution while stirring. The calcium causes coagulation of the alginate and precipitation of 600 mL marine-snow-like flocks. 200 mL of this artificial marine snow was used for each aquaria. Snow+Oil: consist of 200 mL snow, 0.63 g oil and 3.15 g kaolin clay.|||||

Purpose

To understand oil degradation in the settled marine snow and the effect of bioturbation on oil biodegradation in the sediment layer.

Keywords

Oil Biodegradation, Marine Snow

UDI

R4.x267.179:0017

Date

April 2017

Point of Contact

Name

Shokouh Rahsepar

Organization

Wageningen University / Environmental Technology

Name

Albertinka J. Murk

Organization

Wageningen University / Environmental Toxicology and Technology

Funding Source

RFP-4

DOI

10.7266/N7125QPZ

Rights Information

Creative Commons License
This work is licensed under a Creative Commons Public Domain Dedication 1.0 License.

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