Abstract
Lionfish were captured from various locations in the northern Gulf of Mexico from natural and artificial reefs during 2013-03-30 to 2014-03-08. Diet data were collected and analyzed using visual identification and DNA barcoding analyses. This dataset supports the publication: Dahl, K.A., Patterson, W.F., Robertson, A.E., & Ortmann, A.C. (2017). DNA barcoding significantly improves resolution of invasive lionfish diet in the Northern Gulf of Mexico. Biological Invasions, 19, 1917-1933. doi:10.1007/s10530-017-1407-3
Purpose
Observe trends in diet of invasive red lionfish (Pterois volitans) in the northern Gulf of Mexico using DNA barcoding.
Keywords
lionfish, diet, invasive, DNA barcoding
UDI
R4.x267.000:0026
Date
October 2018
Point of Contact
Name
William Frank Patterson
Organization
University of Florida / Fisheries and Aquatic Sciences
Name
Kristen Dahl
Organization
University of South Alabama / Department of Marine Sciences
Funding Source
RFP-4
DOI
10.7266/N7F769ZD
Rights Information
This work is licensed under a
Creative Commons Public Domain Dedication 1.0 License.
Scholar Commons Citation
Kristen Dahl, William Patterson. 2018. DNA barcoding of invasive lionfish diet in the northern Gulf of Mexico, 2013-03-30 to 2014-03-08. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7F769ZD
Comments
Supplemental Information
Site Details worksheet: Site (site location name), Lat (latitude in degrees decimal minutes), Long (longitude in degrees decimal minutes), Habitat (habitat type: AR [artificial reef], NR [natural reef]). Lionfish Sample Data worksheet: Field ID (unique sample number), Date of Capture (date of sample collection), Site (site location name), Species (fish species sampled), Habitat (habitat type: AR [artificial reef], NR [natural reef]), Size Class (size categories by total length: S=small [<200mm TL], M=medium [200-250mm TL], L=large [>250mm TL]), Season (sample timing by season: S=spring, U=summer, F=fall, W=winter), TL (total length, mm), SL (standard length, mm), Mass (kg), Sex (sex of lionfish), Gape Width (mm, lionfish), Gape Height (mm, lionfish), Stomach Mass (weight of stomach, g). Diet Data - Artificial Reefs and Natural Reefs worksheets: Field ID (unique sample number), Prey Taxa (lowest level identification of prey), Prey Family/Order (higher order identification of prey), Prey Category (functional groups of prey), Dry Mass (dry mass of prey, g). Barcode ID (unique sample number for barcoded prey), Field ID (unique sample number), Within Field ID (prey identifier within each individual field sample), Simulated Dry Mass (dry mass simulated from digestion stage and wet/dry conversion factor), Consensus BP (length of prey COI barcoding sequence), Accession Number (unique GenBank identifier of voucher COI sequences), Best Match (GenBank or BOLD closest taxa identification from BLAST search), Taxon (level of identification based on sequence quality and similarity of voucher sequences), Taxon ID (final identification of prey made from DNA barcoding), Common Name (common name of barcoded prey), E-value (measure of sequence match: the number of GenBank search hits with at least this score that you would expect purely by chance), % Identical (% similarity between barcode and voucher sequences), % Grade (measure of sequence match: percentage calculated from query coverage, E-value and % identical), NCBI Accession Number.|||||