Abstract
Sediment samples were collected from the northern Gulf of Mexico (29.3989N, -88.8678W) during an expedition with the R/V Weatherbird on August 21 in 2013, 87 kilometers NW from Macondo wellhead near the Mississippi river Delta at 56 m water depth. Surface (0-5 cm depth) sediment core samples were collected in sterile plastic bags for cultivation and immediately stored at 4˚C for 2 months until enrichment cultures were initiated. Sediment from the 0-5 cm depth interval was homogenized and supplemented as a 10% (w/v) inoculum with sterile anaerobic artificial seawater medium with either hexadecane or phenanthrene.
Purpose
The objectives of this study were (i) to demonstrate hydrocarbon degradation coupled with sulfate reduction and (ii) to characterize the hydrocarbon-degrading microbial community in anoxic sediments of the northern GoM seafloor.
Keywords
Anaerobic, bacteria, 16S rRNA gene amplicon sequencing, 16S rRNA gene cloning, sulfate-reducing, hexadecane, phenanthrene
UDI
R4.x267.000:0094
Date
April 2018
Point of Contact
Name
Joel E. Kostka
Organization
Georgia Institute of Technology / School of Earth and Atmospheric Sciences
Funding Source
RFP-4
DOI
10.7266/N7VD6X0R
Rights Information
This work is licensed under a
Creative Commons Public Domain Dedication 1.0 License.
Scholar Commons Citation
Boryoung Shin, Karsten Zengler, Kuk-Jeong Chin, Will A Overholt, Joel E Kostka. 2018. Anaerobic degradation of hexadecane and phenanthrene coupled to sulfate reduction in seafloor sediments from the northern Gulf of Mexico. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7VD6X0R
Comments
Extent
Dataset contains gene sequencing data using sediment samples collected from the northern Gulf of Mexico (29.3989N, -88.8678W) during an expedition with the R/V Weatherbird on August 21, 2013, 87 kilometers NW from Macondo wellhead near the Mississippi river Delta at 56 m water depth.
Supplemental Information
Sediment samples collected from 56m water depth and 0-5cm surface sediment was used for experiment (Site name : SL1040) Experiment; Library name (name includes hydrocarbon used_DNA/RNA_replicate); MBases; MBytes; Run; SRA sample (SRS2928771); Assay type (AMPLICON); AvgSpotLen (502); BioProject (PRJNA433527); BioSample (SAMN08499993); BioSampleModel (MIMS.me); Consent (public); Insert size (0); Instrument (Illumina MiSeq); Library layout (PAIRED); Library selection (PCR); Library source (GENOMIC); Load date (MM/DD/YYYY); Organism (mixed culture metagenome); Platform (ILLUMINA); Release date (MM/DD/YYYY); SRA study (SRP132511); Sample name (Sulfate-reducing enrichment culture); Sulfate reducing enrichment (mixed culture); Collection date (MM/DD/YYYY); Depth (cm); Elevation (m); Environmental biome (sediment); Environmental material (sediment); Geographic location name (USA); Growth medium (artificial sea water media); Isolation source (Gulf of Mexico); Latitude Longitude (lat_lon, decimal degrees); Petroleum hydrocarbon (Phenanthrene or Hexadecane); Sample type (mixed culture); Carbon source (Phenanthrene and Hexadecane); Concentration (g/L); Sample type (DNA or RNA); Replicate; Duration (161 days of incubation after transfer)|||||