Abstract
Controlled oil exposure study to evaluate the effects of south Louisiana crude oil, via contaminated sediment, on fish health. Data include lipid class composition using Iatroscan of southern flounder exposed via sediment contaminated with south Louisiana crude oil.
Purpose
Controlled oil exposure study to evaluate the effects of south Louisiana crude oil, via contaminated sediment, on fish health.
Keywords
Deepwater Horizon, Flounder, Sediment, Crude oil, Lipid Composition, Paralichthys lethostigma
UDI
R4.x267.000:0068
Date
January 2019
Point of Contact
Name
Dana Wetzel
Organization
Mote Marine Laboratory / Environmental Laboratory of Forensics Program
Funding Source
RFP-4
DOI
10.7266/N718350P
Rights Information
This work is licensed under a
Creative Commons Public Domain Dedication 1.0 License.
Scholar Commons Citation
DL Wetzel, TA Sherwood, CA Miller, RL Medvecky. 2019. Sub-adult Southern flounder exposure to crude oil-contaminated sediment: Lipid Composition and Fatty Acids. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N718350P
Comments
Extent
Dataset contains laboratory measurements of oil exposure on Southern Flounder, no field sampling involved.
Supplemental Information
Test 211 Sample Key worksheet: Collected date (MM/DD/YYYY), Field ID, Tank ID, Matrix, MML Number, Species, Gender, Weight (g), Length (cm). Tank Description worksheet: Tank ID, Sediment Start Date (MM/DD/YYYY), Fish Added Date (MM/DD/YYYY), End Date (MM/DD/YYYY), Treatment, Number of Fish. Sediment Preparation worksheet: Date (MM/DD/YYYY), Time, Tank, Oil (g), Oil ID, Sediment (kg). % Lipid worksheet: Treatment, Fish, MML Liver, Liver % Lipid, MML Whole Body, Whole Body % Lipid. Liver TLC Results worksheet: Treatment, Fish ID, Rep, Liver MML, % Wax esters and Sterol esters, % Triglycerides, % Free Fatty Acids, % Cholesterol, % Phospholipids and Polar Lipids. The "d" on the Liver MML# represents dilution of the same sample (i.e. d2=2x, d5=5x, d10=10x, d50=50x). Whole Body TLC Results worksheet: Treatment, Fish ID, Rep, Whole Fish MML, % Wax esters and Sterol esters, % Triglycerides, % Free Fatty Acids, % Cholesterol, % Phospholipids and Polar Lipids.|Tissue samples were extracted via EPA method 3545 using a Dionex ASE300, then concentrated to 10 mL in DCM for analysis. Lipid Composition: 1 uL of sample analyzed with Iatroscan using PeakSimple 444 software.|Iatroscan MK-6s||QA measures were included into per batch according to Environmental Laboratory for Forensics Quality Assurance Plan. If QA measurements were not met, samples were re-analyzed or data was qualified.|