Abstract
Changes in the ratio of reduced glutathione and oxidized glutathione and total antioxidant power of red drum fed a diet of pellets saturated with south Louisiana crude oil.
Purpose
Controlled oil exposure study to evaluate the effects of south Louisiana crude oil, via contaminated food, on oxidative stress.
Keywords
Diet Exposure, Deepwater Horizon, crude oil, oxidative stress, red drum, Sciaenops ocellatus
UDI
R4.x267.000:0055
Date
January 2019
Point of Contact
Name
Dana Wetzel
Organization
Mote Marine Laboratory / Environmental Laboratory of Forensics Program
Funding Source
RFP-4
DOI
10.7266/N7Q23XRS
Rights Information
This work is licensed under a
Creative Commons Public Domain Dedication 1.0 License.
Scholar Commons Citation
DL Wetzel, TA Sherwood, CA Miller, RL Medvecky. 2019. Red drum diet exposure to Deepwater Horizon crude oil contaminated feed: Oxidative Stress Assays. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N7Q23XRS
Comments
Extent
Dataset contains laboratory measurements of oxidative stress, no field sampling involved.
Supplemental Information
Test 103 Sample Key worksheet: Date Collected (MM/DD/YYYY), Location Collected (MAP - Mote Aquaculture Park), Field ID, Matrix, MML, Species, Project, Tank ID. Food Preparation worksheet: Prep start and end date (MM/DD/YYYY), Est. total mass needed for daily feeding and samples (g), Feed Prep. Method, Feed mass (g), Mass of oil added (g), Mixing Speed, Mixing Time, Oil Source. Oxidative Stress Results worksheet: Treatment, Fish ID, Tank ID, Fish Tag Number, Wt (g), Length (cm), MML, T.A.P (Total Antioxidant Power) Rep 1 (uM), Rep 2 (uM), Average Trolox Concentration (uM), GSH (glutathione uM), GSSG (oxidized glutathione uM), GSH/GSSG (Ratio= (GSHt-2GSSG)/(GSSG)), MDA (malondialdehyde) Rep 1 (uM), Rep 2 (uM), Average (uM).|Sample Collection: plasma 4 mL of whole blood was collected into a sodium citrate blood collection tube. The tube was inverted gently 5 times. The tube was centrifuged at 2000 x g for 10 minutes between 30- 60 minutes from collection time. The supernatant was removed and placed in a clean cryovial and stored in -80degC freezer. Samples were removed and thawed for the first time and are aliquotted out to prevent numerous freeze/thaw cycles. Sample Processing: Samples were run according to the manufacturer's protocol. Total Antioxidant Power Colorimetric Microplate Assay (TA02) by Oxford Biomedical Research, and 2-Thiobarbituric Acid Reactive Substances (FR40) by Oxford Biomedical Research. Sample Collection: whole blood 100ul of whole blood were collected into a microcentrifuge tube containing 30ul of Scavenger. 50ul of whole blood were collected into an empty microcentrifuge tube. Tubes were gently inverted and stored in -80degC freezer or in LN2. Sample Processing: Samples assayed according to the manufacturer's protocol. Total Antioxidant Power Colorimetric Microplate Assay (TA02) by Oxford Biomedical Research, GSH/GSSG Microplate Assay (GT40) by Oxford Biomedical Research.|DS2 Automated ELISA instrument by Dynex Technologies.||%CV for the standard curve less than 25% is acceptable, less than 15% for sample replicates.|