Abstract
Vitellogenin is an egg-yolk precursor protein expressed in females of nearly all oviparous species and is commonly used to detect estrogenic effects in male fish. Vitellogenin antibodies were validated for each of the collected fish species and will be analyzed used appropriate antibodies. The vitellogenin assays-concentrations will be reported as ng/ml of plasma.
Purpose
Assessment of vitellogenin levels in plasma to determine liklihood of endocrine disruption.
Keywords
Vitellogenin, Fertility, Hormone, Health assessment, Red snapper, Golden tilefish, Plasma
UDI
R1.x135.121:0007
Date
July 2016
Point of Contact
Name
Dana Wetzel
Organization
Mote Marine Laboratory / Environmental Laboratory of Forensics Program
Funding Source
RFP-1
DOI
10.7266/N71C1TWG
Rights Information
This work is licensed under a
Creative Commons Public Domain Dedication 1.0 License.
Scholar Commons Citation
Wetzel, Dana. 2016. Plasma vitellogenin levels of collected fish species, DeSoto Canyon, August 16-29, 2013. Distributed by: Gulf of Mexico Research Initiative Information and Data Cooperative (GRIIDC), Harte Research Institute, Texas A&M University–Corpus Christi. doi:10.7266/N71C1TWG
Comments
Supplemental Information
Plate (ID), Assay (ID), Curve Fit Type [linear regression, R-squared], Well Positions (ID), Sample (ID), Raw Data, Curve Fit, Final Results, Sample (ID), Sex (M,F), Species (common name) [Red Snapper, Golden tilefish], Vitellogenin Concentration Rep 1 to Rep 3 (ng/mL), Vitellogenin Concentration Average (ng/mL), Vitellogenin Concentration Standard Deviation, Vitellogenin Concentration % CV coefficient variation of absorbance (%). The following is a list of stations where samples in the dataset were collected from (Station ID, Westbound Extent, Northbound Extent, Southbound Extent, Eastbound Extent): WB-SL10-40, -88.52829, 29.12272, 29.08907, -88.52213; WB-HE-265, -90.30623, 28.23532, 28.22594, -90.28127; WB-SL16-150, -90.00278, 28.3727, 28.3387, -89.3387; WB-SL12-100, -89.35517, 28.3347, 28.32427, -89.33911; WB-SL11-150, -88.37047, 29.03165, 29.02626, -88.3441; WB-SL5-100, -85.26157, 28.3284, 28.32743, -85.22738; WB-SL4-40, -84.25941, 28.07729, 28.05337, -84.24252; The dataset file titled " Vitellogen 2013 Sample Locations" contains information about where each sample in the dataset was collected from and is organized by Plate ID. The following is a description of each field in the file: Fish Sample ID (unique identifier that corresponds to the Sample ID fields in the other dataset files), Station ID (station where sample was collected), Westbound Extent (western bounding extent of station (DD)), Northbound Extent (northern bounding extent of station (DD)), Southbound Extent (southern bounding extent of station (DD)), Eastbound Extent (eastern bounding extent of station (DD)), Center Point Lat (latitudinal center point of station bounding extent(DD)), Center Point Lon (longitudinal center point of station bounding extent (DD)). |Sample Collection: plasma - Collect 4 mL whole blood into a green top lithium heparin tube containing aprotinin. - Invert the tube 5 times and allow to clot on ice for 30 minutes. - Spin at 2700 rpm for 15 minutes. - Remove supernatant and place in green top cryovial containing aprotinin. - Store in -20 freezer or in LN2 Sample Processing: Samples are run according to manufacturer's protocol|DS2 Automated ELISA instrument by Dynex Technologies Vitellogenin Rainbow Trout ELISA by Biosense Laboratories||Standard curves and no substrate blanks are run with each plate Samples are run in triplicate Sample %CV's are calculated and outliers are removed such that %CV is less than 20%|