Orbitrap Mass Spectrometry for Analysis of Stable Isotopes in Trace Concentrations of Amino Acids without Separation Methods
Document Type
Data
Publication Date
2024
Abstract
Using an Orbitrap mass spectrometer for stable isotope ratio measurements, as an alternative to traditional isotope ratio mass spectrometry (IRMS), can reduce required mass of sample and use of chromatographic separation. Here we demonstrate the use of an Orbitrap in determining the isotope ratio of amino acids. Nitrogen and carbon standards were analyzed and reported under ± 0.4 ‰ SE (standard error) and ± 0.3 SE respectively. Analyte sample data reported under ± 0.3 ‰ SE and ± 0.1 SE. Additionally, measurements of amino acids extracted from mollusk shells demonstrate the instrument's ability to resolve isotope ratios from a crude extract without separation. These data suggest that large scale food web and geological time scale analysis can be performed on an Orbitrap, for analysis requiring fine scale this method requires more work to perform at the same precision that is available via IRMS.
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Citation / Publisher Attribution
Orbitrap Mass Spectrometry for Analysis of Stable Isotopes in Trace Concentrations of Amino Acids without Separation Methods, Supplementary Files
Scholar Commons Citation
Keidel, Alexandra, "Orbitrap Mass Spectrometry for Analysis of Stable Isotopes in Trace Concentrations of Amino Acids without Separation Methods" (2024). Chemistry Faculty Publications. 227.
https://digitalcommons.usf.edu/chm_facpub/227
Supplemental File 1
Stable Isotope Ratios Amino Acids Digital Commons.xlsx (21 kB)
Supplemental File 2
