College
College of Arts & Sciences
Mentor Information
Kirpal Bisht
Description
Lectins are proteins that interact with sugars on cell membranes and bind with known affinities. Isothermal Titration Calorimetry (ITC) can give insight into entropy (ΔS), enthalpy (ΔH), stoichiometry (n) and the dissociation constant, KD. Resorcin[4]arene cavitand glycoconjugates were synthesized by attaching one of three sugar derivatives of glucose, mannose, and lactose to each of the cavitands for the purpose of comparing binding strength for concanavalin A between the glycosides and their simple sugar. The glycoconjugate structure differs from that of glycans, and therefore the differences in interactions between the sugars on the cavitands must be investigated. The multivalent resorcinarene glycoconjugates were synthesized and characterized using NMR spectroscopy. Project outcomes will provide insight into lectin-glycoconjugate binding strength for potential applications in biosensing and biomolecular engineering.
Glycoconjugate Binding: Lectin Affinity for Structurally Differing Sugars on Cavitands
Lectins are proteins that interact with sugars on cell membranes and bind with known affinities. Isothermal Titration Calorimetry (ITC) can give insight into entropy (ΔS), enthalpy (ΔH), stoichiometry (n) and the dissociation constant, KD. Resorcin[4]arene cavitand glycoconjugates were synthesized by attaching one of three sugar derivatives of glucose, mannose, and lactose to each of the cavitands for the purpose of comparing binding strength for concanavalin A between the glycosides and their simple sugar. The glycoconjugate structure differs from that of glycans, and therefore the differences in interactions between the sugars on the cavitands must be investigated. The multivalent resorcinarene glycoconjugates were synthesized and characterized using NMR spectroscopy. Project outcomes will provide insight into lectin-glycoconjugate binding strength for potential applications in biosensing and biomolecular engineering.
