Automatic stereological analysis of biological tissue including section thickness determination

Authors

Kurt A. Kramer
Peter R. Mouton
Lawrence O. Hall
Dmitry Goldgof
Daniel T. Elozory
Om Pavithra Bonam
Baishali Chaudhury

Document Type

Patent

Publication Date

3-29-2016

Patent Number

9297995

CPC

G02B 21/0004 (20130101)

Abstract

Systems and methods are provided for automatic determination of slice thickness of an image stack in a computerized stereology system, as well as automatic quantification of biological objects of interest within an identified slice of the image stack. Top and bottom boundaries of a slice can be identified by applying a thresholded focus function to determine just-out-of-focus focal planes. Objects within an identified slice can be quantified by performing a color processing segmentation followed by a gray-level processing segmentation. The two segmentation processes generate unique identifiers for features in an image that can then be used to produce a count of the features.

Application Number

13/371,889

Recommended Citation

Kramer, Kurt A.; Mouton, Peter R.; Hall, Lawrence O.; Goldgof, Dmitry; Elozory, Daniel T.; Bonam, Om Pavithra; and Chaudhury, Baishali, "Automatic stereological analysis of biological tissue including section thickness determination" (2016). USF Patents. 843.
https://digitalcommons.usf.edu/usf_patents/843

Assignees

University of South Florida

Filing Date

2012-02-13