Method of determining melanoma micrometastasis using tyrosinase

Authors

Douglas S. Reintgen

Document Type

Patent

Publication Date

November 2000

Patent Number

61533882

Abstract

A highly sensitive method to detect melanoma micrometastasis by examining lymph nodes for the presence of tyrosinase messenger RNA. In a preferred mode, this is accomplished using the combination of reverse transcription and double round polymerase chain reaction (RT-PCR). The amplified samples are examined on a 2% agarose gel and tyrosinase is seen as a 207 base pair fragment. The lymph nodes examined are determined using pre- and intra- operative node mapping.

Application Number

09/245,822

Recommended Citation

Reintgen, Douglas S., "Method of determining melanoma micrometastasis using tyrosinase" (2000). USF Patents. 819.
https://digitalcommons.usf.edu/usf_patents/819

Assignees

University of South Florida

Filing Date

02/05/1999

Primary/U.S. Class

435/6