A DNA expression vector for positively selecting in-frame or out-of-reading-frame mutations in DNA sequences to be tested comprising a promotor operatively linked to an expressible reporter gene through a linkage sequence is disclosed. The linkage sequence includes at least two restriction sites and an engineered frameshift mutation. In an embodiment the frameshift is established by complementary sequences SEQ ID Nos:1 and 2. The expressible reporter gene is expressed as a fusion product including a green fluorescent protein and the promoter can be lacZ and inducible in E. coli.
Litman, Gary W.; Hawke, Noel A.; Haire, Robert N.; and Strong, Scott J., "DNA vector for determining the presence of out-of-reading-frame mutations" (2001). USF Patents. 810.
University of South Florida