DNA vector for determining the presence of out-of-reading-frame mutations

Authors

Gary W. Litman
Noel A. Hawke
Robert N. Haire
Scott J. Strong

Document Type

Patent

Publication Date

September 2001

Patent Number

62844962

Abstract

A DNA expression vector for positively selecting in-frame or out-of-reading-frame mutations in DNA sequences to be tested comprising a promotor operatively linked to an expressible reporter gene through a linkage sequence is disclosed. The linkage sequence includes at least two restriction sites and an engineered frameshift mutation. In an embodiment the frameshift is established by complementary sequences SEQ ID Nos:1 and 2. The expressible reporter gene is expressed as a fusion product including a green fluorescent protein and the promoter can be lacZ and inducible in E. coli.

Application Number

09/165,934

Recommended Citation

Litman, Gary W.; Hawke, Noel A.; Haire, Robert N.; and Strong, Scott J., "DNA vector for determining the presence of out-of-reading-frame mutations" (2001). USF Patents. 810.
https://digitalcommons.usf.edu/usf_patents/810

Assignees

University of South Florida

Filing Date

01/02/1998

Primary/U.S. Class

435/912