Document Type
Patent
Publication Date
February 2001
Patent Number
61908843
Abstract
The invention relates to a method of producing connective tissue growth factor (CTGF). A method of the invention can be performed, for example, by transforming a host cell with a polynucleotide encoding a CTGF polypeptide having an amino acid sequence of SEQ ID NO: 2 or an active fragment thereof; and growing the host cells under conditions suitable for expressing the polynucleotide in the host cell. In an embodiment, the method further involves isolating the CTGF polypeptide or the active fragment. The invention also relates to a method for producing a vector containing a polynucleotide encoding a CTGF polypeptide or an active fragment thereof by inserting a polynucleotide encoding a CTGF polypeptide having an amino acid sequence of SEQ ID NO: 2 or a variant thereof into the vector. The vector can be an expression vector, for example, a plasmid expression vector or a viral expression vector. In an embodiment, the polynucleotide has a nucleotide sequence of SEQ ID NO: 1 or a variant thereof.
Application Number
09/080,715
Recommended Citation
Grotendorst, Gary R. and Bradham, Douglas Douglas M., "Connective tissue growth factor" (2001). USF Patents. 806.
https://digitalcommons.usf.edu/usf_patents/806
Assignees
University of South Florida
Filing Date
05/18/1998
Primary/U.S. Class
435/694
