Document Type
Patent
Publication Date
June 2010
Patent Number
7745168
Abstract
The present invention provides a method for the staining of fungi and microsporidia for observation with a light microscope based upon the presence of chitin in the composition of these organisms. With the method of the present invention a sample to be analyzed is treated with a solution of Ponceau S and Stains-all dye. The sample is then selectively decolorized and rinsed. The resulting sample is examined with a light microscope, or photographed for a permanent record, to identify the presence of a variety of microorganisms, to include fungi and microsporidia.
Application Number
11/537,665
Recommended Citation
Dao, My Lien, "Method for selectively staining chitin-containing organisms" (2010). USF Patents. 497.
https://digitalcommons.usf.edu/usf_patents/497
Assignees
University of South Florida
Filing Date
10/02/2006
Primary/U.S. Class
435/29
