Document Type
Patent
Publication Date
August 2015
Patent Number
9116151
CPC
G01N 33/56916(20130101), G01N 33/56938(20130101), G01N 33/56944(20130101)
Abstract
Disclosed is a method and associated device for the rapid identification of viable bacterial contaminants in food products. The method detects viable microbes by using a combined ATP-bioluminescence immunoassay. Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium were selected as target organisms in various matrices including ground beef homogenate, apple juice, milk, and phosphate-buffered saline. Specific antibodies were immobilized on the surface of well plates in which the sample matrices were incubated. The plates were washed, and the wells were incubated with BacTiter-Glo reagent in Mueller-Hinton II broth. Bioluminescent output was measured with a luminometer and signal-to-noise ratios were calculated. The LOD was not affected by the presence of non-target cells. A strong linear correlation was observed between the number of cells and luminescent output over 4 orders of magnitude. This method provides a means of simultaneously detecting and identifying viable pathogens in complex matrices.
Application Number
13/952,193
Recommended Citation
Lim, Daniel V. and Hunter, Dawn M., "Detection plate for ATP-bioluminescence immunoassay and method of manufacturing" (2015). USF Patents. 49.
https://digitalcommons.usf.edu/usf_patents/49
Assignees
University of South Florida
Filing Date
07/26/2013
