Document Type
Patent
Publication Date
August 2014
Patent Number
8796235
Abstract
The present invention pertains to vectors for regulating gene expression having at least one gene expressing cassette and at least one gene suppressing cassette, wherein the gene expression cassette encodes a polypeptide of interest, and wherein the gene suppressing cassette encodes a short interfering RNA (siRNA) molecule that reduces expression of a target gene by RNA interference. The present invention further includes vectors that contain suppressor cassettes in conjunction with cassettes upregulating gene expression regulated by either a constitutive promoter, such as a general CMV promoter, or a tissue specific promoter. The present invention further includes vectors that contain Dengue virus gene suppression cassettes. The present invention further includes pharmaceutical compositions containing such vectors, methods of modulating the expression of genes in a host using such vectors, and method of producing such vectors.
Application Number
10/544,146
Recommended Citation
Mohapatra, Shyam S. and Zhang, Weidong, "Methods for attenuating dengue virus infection" (2014). USF Patents. 145.
https://digitalcommons.usf.edu/usf_patents/145
Assignees
University of South Florida
Filing Date
02/23/2004
Primary/U.S. Class
514/44A
