Document Type
Article
Publication Date
December 2021
Patent Number
11198842
CPC
C12M 23/12, C12M 25/02, C12M 23/16
Abstract
An in vitro microfluidic device includes a device configured to model a blood-brain barrier. The device includes a center well in fluidic communication with each of an inlet and an outlet. Each of the center well, inlet, and outlet includes a porous membrane that separates a âbloodâ portion (a fluid flow portion) from a âbrainâ portion (a fluid containing portion). The porous membrane is seeded with endothelial cells such as the human venule endothelial cells (HUVECs) on the blood side, and with astrocytes on the brain side, to accurately model the blood-brain barrier. Fluid flows between the inlet, the center well, and the outlet to test the permeability of the porous membrane, thereby providing an accurate in vitro model of a blood-brain barrier.
Application Number
16/799428
Recommended Citation
Mohapatra, Subhra; Mohapatra, Shyam S.; Wang, Tao; and Martinez, Taylor, "Microfluidic-coupled in vitro model of the blood-brain barrier" (2021). USF Patents. 1280.
https://digitalcommons.usf.edu/usf_patents/1280
Assignees
University of South Florida
Filing Date
02/24/2020
