Document Type
Article
Publication Date
February 2020
Patent Number
10551389
CPC
G01N 33/6848
Abstract
Methods of identifying target binding molecules by target guided synthesis are provided. The methods include providing two or more fragments capable of reacting to form the target binding molecule and mixing the fragments with the target. The methods can be used to identify target binding molecules that bind targets such as proteins or nucleic acids, including those that bind shallow binding pockets on the surface of such targets. The methods are applied to the Bcl-XL and Mcl-1 proteins from the Bcl-2 family of proteins. Using thio acid and sulfonyl azide fragments capable of reacting through sulfo-click chemistry, new acyl sulfonamides are identified that bind one or both of the Bcl-XL and Mcl-1 proteins. Pharmaceutical formulations of these target binding molecules are also provided.
Application Number
14/889443
Recommended Citation
Manetsch, Roman; Nacheva, Katya Pavlova; Flanigan, David Lawrence; Namelikonda, Niranjan Kumar; Iyamu, Iredia David; Kulkarni, Sameer Shamrao; Barber, Megan M.; Tipton, Jeremiah Dwayne; Wang, Hong-Gang; and Doi, Kenichiro, "Target binding molecules identified by kinetic target-guided synthesis" (2020). USF Patents. 1123.
https://digitalcommons.usf.edu/usf_patents/1123
Assignees
University of South Florida
Filing Date
08/06/2014
