Sequence Comparison of Avian Infectious Bronchitis Virus S1 Glycoproteins of the Florida Serotype and Five Variant Isolates from Georgia and California

Authors

Kristi M. Moore, Southeast Poultry Research Laboratory
Joyce D. Bennett, Southeast Poultry Research Laboratory
Bruce S. Seal, Southeast Poultry Research Laboratory
Mark W. Jackwood, University of Delaware

Document Type

Article

Publication Date

1-1998

Keywords

Amino Acid Sequence, Animals, Base Sequence, California, Chickens, Florida, Genetic Variation, Georgia, Infectious bronchitis virus, Membrane Glycoproteins, Molecular Sequence Data, Phylogeny, Polymorphism, Restriction Fragment Length, Reverse Transcriptase Polymerase Chain Reaction, Sequence Alignment, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Serotyping, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins

DOI

https://doi.org/10.1023/A:1008057118625

Abstract

The infectious bronchitis virus (IBV) spike glycoprotein S1 subunit is required to initiate infection and contains virus-neutralizing and serotype-specific epitope(s). Reported are the S1 gene nucleotide and predicted amino acid sequences for the Florida 18288 strain and isolates GA-92, CV-56b, CV-9437, CV-1686, and 1013. These sequences were compared with previously published gene sequences of IBV strains, and phylogenetic relationships are reported. The S1 amino acid sequence of Florida 18288 was 94.9% similar to the Connecticut strain, and GA-92 was 92.8% similar to the Arkansas 99 strain. S1 amino acid sequences of the California variants, CV-56b, CV-9437, and CV-1686, were 97.6-99.3% similar to one another and only 76.6%-76.8% similar to the Arkansas-type strains. Isolate 1013, also from California, was 84.0% similar to Ark DPI and 77.9% similar to CV-56b. When comparing 19 viruses isolated from the United States, sequence variations were observed between amino acids 55-96, 115-149, 255-309, and 378-395. Similar regions are reported to be involved in virus-neutralizing and/or serotype-specific epitopes. These data demonstrate that variant IBV strains continue to emerge, and unique variants may circulate among poultry in geographically isolated areas.

Comments

Article available for free at PubMed Central: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/9778790/

Citation / Publisher Attribution

Virus Genes, v. 17, issue 1, p. 63-83

Scholar Commons Citation

Moore, Kristi M.; Bennett, Joyce D.; Seal, Bruce S.; and Jackwood, Mark W., "Sequence Comparison of Avian Infectious Bronchitis Virus S1 Glycoproteins of the Florida Serotype and Five Variant Isolates from Georgia and California" (1998). All publications. 133.
https://digitalcommons.usf.edu/usf_fcrc_all/133