Tip-enhanced Raman Scattering of DNA Aptamers for Listeria monocytogenes
Document Type
Article
Publication Date
2018
Digital Object Identifier (DOI)
https://doi.org/10.1116/1.5022303
Abstract
Optical detection and conformational mapping of aptamers are important for improving medical and biosensing technologies and for better understanding of biological processes at the molecular level. The authors investigate the vibrational signals of deoxyribonucleic acid aptamers specific to Listeria monocytogenes immobilized on gold substrates using tip-enhanced Raman scattering (TERS) spectroscopy and nanoscale imaging. The authors compare topographic and nano-optical signals and investigate the fluctuations of the position-dependent TERS spectra. They perform spatial TERS mapping with 3 nm step size and discuss the limitation of the resulting spatial resolution under the ambient conditions. TERS mapping provides information about the chemical composition and conformation of aptamers and paves the way to future label-free biosensing.
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
Biointerphases, v. 13, art. 03C402
Scholar Commons Citation
He, Siyu; Li, Hongyuan; Gomes, Carmen L.; and Voronin, Dmitri V., "Tip-enhanced Raman Scattering of DNA Aptamers for Listeria monocytogenes" (2018). Physics Faculty Publications. 61.
https://digitalcommons.usf.edu/phy_facpub/61
