Document Type
Article
Publication Date
2019
Keywords
apoptosis, eIF3c, IPA, ovarian cancer cells, proliferation
Digital Object Identifier (DOI)
https://doi.org/10.1042/BSR20191124
Abstract
Ovarian cancer remains the leading cause of death among all gynaecological cancers, illustrating the urgent need to understand the molecular mechanisms involved in this disease. Eukaryotic initiation factor 3c (EIF3c) plays an important role in protein translation and cancer cell growth and proliferation, but its role in human ovarian cancer is unclear. Our results showed that EIF3c silencing significantly up-regulated 217 and down-regulated 340 genes. Ingenuity Pathway Analysis (IPA) indicated that the top differentially expressed genes are involved in ‘Classical Pathways’, ‘Diseases and Functions’ and ‘Networks’, especially those involved in signalling and cellular growth and proliferation. In addition, eIF3c silencing inhibited cellular proliferation, enhanced apoptosis and regulated the expression of apoptosis-associated proteins. In conclusion, these results indicate that by dysregulating translational initiation, eIF3c plays an important role in the proliferation and survival of human ovarian cancer cells. These results should provide experimental directions for further in-depth studies on important human ovarian cancer cell pathways.
Rights Information
This work is licensed under a Creative Commons Attribution 4.0 License.
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
BioScience Reports, v. 39, issue 8, art. BSR20191124
Scholar Commons Citation
Wen, Fang; Wu, Zhang-Ying; Nie, Lei; Zhang, Qi-Zhu; Qin, Yuan-Kun; Zhou, Zun-lun; Wu, Jin-Jian; Zhao, Xing; Tan, Jun; Sawmiller, Darrell; and Zi, Dan, "Eukaryotic Initiation Factor 3, Subunit C Silencing Inhibits Cell Proliferation and Promotes Apoptosis in Human Ovarian Cancer Cells" (2019). Psychiatry & Behavioral Neurosciences Faculty Publications. 2.
https://digitalcommons.usf.edu/pbn_facpub/2
