Structures of the Wild-type and S59L Mutant CHCHD10 Proteins Important in Amyotrophic Lateral Sclerosis–frontotemporal Dementia

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CHCHD10, Wild-type, S59L Genetic Mutation, Amyotrophic Lateral Sclerosis-frontotemporal Dementia.

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The S59L genetic mutation of the mitochondrial coiled-coil–helix-coiled–coil-helix domain-containing protein 10 (CHCHD10) is involved in the pathogenesis of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The wild-type and mutant forms of this protein contain intrinsically disordered regions, and their structural characterization has been facing challenges. Here, for the first time in the literature, we present the structural ensemble properties of the wild-type and S59L mutant form of CHCHD10 in an aqueous solution environment at the atomic level with dynamics. Even though available experiments suggested that the S59L mutation may not change the structure of the CHCHD10 protein, our structural analysis clearly shows that the structure of this protein is significantly affected by the S59L mutation. We present here the secondary structure components with their abundances per residue, the tertiary structure properties, the free energy surfaces based on the radius of gyration and end-to-end distance values, the Ramachandran plots, the quantity of intramolecular hydrogen bonds, and the principal component analysis results. These results may be crucial in designing more efficient treatment for ALS and FTD diseases.

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ACS Chemical Neuroscience, v. 13, issue 8, p. 1273-1280